Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	174.4 / NA	38	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (27/11);  total (27/11)

CCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765141‑765234                                                 |                                              ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:1822727/1‑49 (MQ=255)      tttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                        >  1:1472839/1‑50 (MQ=39)        tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      >  1:2572557/1‑50 (MQ=39)        tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      >  1:2298449/1‑50 (MQ=39)           gCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                   >  1:2534240/1‑50 (MQ=39)            cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:529010/1‑50 (MQ=39)              tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                                >  1:1279242/1‑50 (MQ=39)              tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                                >  1:202256/1‑50 (MQ=39)               aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                 <  1:2099331/48‑1 (MQ=38)                tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                               <  1:2288248/49‑1 (MQ=38)                tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                               <  1:1695573/49‑1 (MQ=38)                   cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAta                           <  1:311374‑M1/50‑3 (MQ=255)                    gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:167373‑M1/1‑47 (MQ=255)                     atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                         <  1:2140299‑M1/50‑5 (MQ=255)                        atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      >  1:1844422‑M1/1‑43 (MQ=255)                         ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                     <  1:410685‑M1/50‑9 (MQ=255)                         ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                     <  1:1263530‑M1/50‑9 (MQ=255)                         ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      >  1:1771179‑M1/1‑42 (MQ=255)                          tttCGCTGCGCTATCCGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                     >  1:2340897‑M1/1‑41 (MQ=255)                          tttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                     >  1:578162‑M1/1‑41 (MQ=255)                             cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:963074‑M1/1‑38 (MQ=255)                             cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:2659194‑M1/1‑38 (MQ=255)                             cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:1706871‑M1/1‑38 (MQ=255)                             cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  <  1:678574‑M1/49‑12 (MQ=255)                              gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                <  1:2512072‑M1/50‑14 (MQ=255)                              gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                <  1:1651447‑M1/50‑14 (MQ=255)                                 gcgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               >  1:1603340‑M1/1‑34 (MQ=255)                                 gcgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               >  1:1415707‑M1/1‑34 (MQ=255)                                  cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaag            >  1:1394880‑M1/1‑33 (MQ=255)                                  cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaag            <  1:1738243‑M1/50‑18 (MQ=255)                                  cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaag            >  1:349574‑M1/1‑33 (MQ=255)                                        cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       >  1:248087‑M1/1‑27 (MQ=255)                                        cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       >  1:2629528‑M1/1‑27 (MQ=255)                                         aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata     >  1:1045023‑M1/1‑26 (MQ=255)                                         aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata     >  1:1987153‑M1/1‑26 (MQ=255)                                          gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat    >  1:2382705‑M1/1‑25 (MQ=255)                                          gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat    >  1:232973‑M1/1‑25 (MQ=255)                                           ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg   >  1:599784‑M1/1‑24 (MQ=255)                                            gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatgc  >  1:1242367‑M1/1‑23 (MQ=255)                                                 |                                              CCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765141‑765234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑