Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	972,866	Δ1 bp	coding (536/780 nt)	elyC ←	envelope biogenesis factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	972,863	0	G	.	100.0%	77.2 / NA	19	coding (539/780 nt)	elyC	envelope biogenesis factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (10/9);  total (10/9)

AAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGG  >  NC_000913/972815‑972908                                                 |                                              aaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgg                                              >  1:2564358/1‑50 (MQ=255) aaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgg                                              >  1:2221377/1‑50 (MQ=255)          gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCa                                    <  1:1078597/50‑1 (MQ=39)             cgcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATc                                 >  1:161614/1‑50 (MQ=39)             cgcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATc                                 <  1:2301827/50‑1 (MQ=39)             cgcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATc                                 >  1:2676913/1‑50 (MQ=39)                   aGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGt                           >  1:2571508/1‑50 (MQ=39)                    gATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGtt                          >  1:1602062/1‑50 (MQ=39)                      tgtgATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGttt                         >  1:1794178/1‑49 (MQ=39)                      tgtgATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTc                        <  1:2173223/50‑1 (MQ=39)                          aTGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActg                    <  1:872257/50‑1 (MQ=39)                              ggAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActgctg                 >  1:1595042/1‑49 (MQ=39)                               gAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCa               <  1:887614/50‑1 (MQ=39)                                  gTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGct            >  1:646009/1‑50 (MQ=39)                                      ccAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttctt        <  1:1832866/50‑1 (MQ=39)                                      ccAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttctt        <  1:783776/50‑1 (MQ=39)                                        agcagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttcttct      >  1:2336591/1‑50 (MQ=39)                                          cagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCttcttcttc    >  1:996969/1‑50 (MQ=39)                                          cagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCttcttcttc    <  1:1304974/50‑1 (MQ=39)                                            ggAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCgg  >  1:386577/1‑50 (MQ=39)                                            ggAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCgg  <  1:1479183/50‑1 (MQ=39)                                                 |                                              AAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGG  >  NC_000913/972815‑972908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑