Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,720,597	Δ1 bp	coding (4/153 nt)	hokA ←	small toxic polypeptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,720,596	0	G	.	100.0%	177.2 / NA	37	coding (5/153 nt)	hokA	small toxic polypeptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (12/25);  total (12/25)

TAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCA  >  NC_000913/3720547‑3720641                                                  |                                              tAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCg                                               >  1:813884/1‑50 (MQ=255)   aaaGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCgca                                             >  1:719071/1‑48 (MQ=255)        gTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTccc                                       <  1:2529522/50‑1 (MQ=255)         tGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCa                                      <  1:1753307/50‑1 (MQ=255)             aCAAATCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcct                                  <  1:1518525/50‑1 (MQ=255)               aaaTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctcc                                >  1:1615090/1‑50 (MQ=255)               aaaTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctcc                                >  1:172680/1‑50 (MQ=255)               aaaTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctcc                                <  1:1741720/50‑1 (MQ=255)                aaTCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGcctcct                               <  1:624808/50‑1 (MQ=255)                  tCACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgt                             <  1:2435044/50‑1 (MQ=255)                   cACTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCtgtg                            >  1:2463831/1‑50 (MQ=255)                    aCTATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTgtgt                           <  1:1512229/50‑1 (MQ=255)                      tATTAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGtt                          >  1:333124/1‑49 (MQ=255)                        ttAAAGAAAGTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTg                         <  1:2244311/48‑1 (MQ=255)                        ttAAAGAAAGTAAACTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTg                         <  1:1166461/48‑1 (MQ=255)                          aaagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGAtt                      <  1:2612094/49‑1 (MQ=255)                           aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc                    >  1:1802538/1‑50 (MQ=255)                           aagaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTc                    <  1:1000288/50‑1 (MQ=255)                            agaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc                   <  1:1717994/50‑1 (MQ=255)                            agaaagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTcc                   <  1:2578791/50‑1 (MQ=255)                               aagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc                <  1:543358/50‑1 (MQ=255)                               aagTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAAc                <  1:538237/50‑1 (MQ=255)                                agTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACg               <  1:1768458/50‑1 (MQ=255)                                agTAATCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACg               <  1:858539/50‑1 (MQ=255)                                 gTAATCTATATTTCTGC‑GCATTCCCAGCGTCCTGTGTTGATTTCCAAc                >  1:1703429/1‑48 (MQ=255)                                    aTCTATATTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGt            <  1:1435899/49‑1 (MQ=255)                                        atatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaac       >  1:2700254‑M1/1‑47 (MQ=255)                                        atatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaac       >  1:414739‑M1/1‑47 (MQ=255)                                         tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTAt          <  1:1854858/46‑1 (MQ=255)                                         tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaact      <  1:966256‑M1/50‑5 (MQ=255)                                         tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaact      >  1:1867749‑M1/1‑46 (MQ=255)                                         tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaact      <  1:1299270‑M1/50‑5 (MQ=255)                                         tatTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaa        <  1:1508812‑M1/48‑3 (MQ=255)                                          atTTCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactt     >  1:1615825‑M1/1‑45 (MQ=255)                                           tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaacttt    >  1:2010788‑M1/1‑44 (MQ=255)                                           tttCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactt     <  1:397845‑M1/49‑6 (MQ=255)                                             tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactttga  >  1:342087‑M1/1‑42 (MQ=255)                                             tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactttg   <  1:2695457‑M1/49‑8 (MQ=255)                                             tCTGC‑GCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATaactttg   <  1:2200624‑M1/49‑8 (MQ=255)                                                  |                                              TAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCA  >  NC_000913/3720547‑3720641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑