Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	87.6 / NA	25	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/9);  total (16/9)

CGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988453‑988545                                               |                                               cgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                                             >  1:2690701/1‑50 (MQ=255)  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            <  1:1607646/50‑1 (MQ=255)  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:2038427/1‑50 (MQ=255)   gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:488541/1‑49 (MQ=255)     aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                         <  1:2386777/50‑1 (MQ=255)      cATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaaa                                        <  1:2411108/50‑1 (MQ=255)          tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                     <  1:2532825/49‑1 (MQ=255)            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCaga                                  <  1:125873/50‑1 (MQ=255)                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaaga                               <  1:1684076/48‑1 (MQ=255)                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                             >  1:958073/1‑50 (MQ=255)                    cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          >  1:2159922/1‑50 (MQ=255)                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         <  1:304777/48‑1 (MQ=255)                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                       >  1:1441057/1‑50 (MQ=255)                        ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                      >  1:2305020/1‑50 (MQ=255)                         gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                     >  1:1112807/1‑50 (MQ=255)                          tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                    >  1:1526753/1‑50 (MQ=255)                             cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  >  1:1990183/1‑49 (MQ=255)                             cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  >  1:398703/1‑49 (MQ=255)                                     gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  1:418260/1‑49 (MQ=255)                                      tAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGa        <  1:2674305/50‑1 (MQ=255)                                      tAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGa        >  1:287911/1‑50 (MQ=255)                                          cAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  1:545404/1‑48 (MQ=255)                                           aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCa    <  1:2442239/49‑1 (MQ=255)                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  >  1:165675/1‑50 (MQ=255)                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  >  1:1874229/1‑50 (MQ=255)                                               |                                               CGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988453‑988545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑