Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,362	C→T	A84V (GCC→GTC)	mltG →	endolytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,362	0	C	T	100.0%	124.0 / NA	35	A84V (GCC→GTC)	mltG	endolytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/19);  total (16/19)

TTTTCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATG  >  NC_000913/1154315‑1154405                                                |                                            ttttCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCg                                           >  1:2535420/1‑50 (MQ=255)  tttCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCgg                                          >  1:1653211/1‑50 (MQ=255)       aTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACtt                                     <  1:1970250/50‑1 (MQ=255)       aTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACt                                      >  1:1825076/1‑49 (MQ=255)          gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                  <  1:608012/50‑1 (MQ=255)          gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                  <  1:1617266/50‑1 (MQ=255)           ctgctgCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCg                                 <  1:1760680/50‑1 (MQ=255)              ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGc                                <  1:1164909/48‑1 (MQ=255)              ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCt                               <  1:377955/49‑1 (MQ=255)                 cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTa                            >  1:1879052/1‑49 (MQ=255)                     tCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACAcc                        <  1:1017161/49‑1 (MQ=255)                     tCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACAcc                        <  1:1846600/49‑1 (MQ=255)                       gAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                       >  1:1482495/1‑48 (MQ=255)                       gAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                       >  1:344334/1‑48 (MQ=255)                        aaCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                       >  1:506683/1‑47 (MQ=255)                        aaCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAg                    <  1:243378/50‑1 (MQ=255)                         aCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                       >  1:492296/1‑46 (MQ=255)                         aCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGa                   <  1:144595/50‑1 (MQ=255)                          ccGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                  >  1:475803/1‑50 (MQ=255)                           cGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                  >  1:981407/1‑49 (MQ=255)                           cGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATg                 <  1:186598/50‑1 (MQ=255)                                cTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGTAGATGACCGt            >  1:234861/1‑50 (MQ=255)                                cTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt            >  1:2184068/1‑50 (MQ=255)                                 tttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTg           >  1:1017277/1‑50 (MQ=255)                                   tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc              <  1:2345122/45‑1 (MQ=255)                                   tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc              <  1:2063728/45‑1 (MQ=255)                                   tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc              <  1:1241113/45‑1 (MQ=255)                                   tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc              <  1:1152091/45‑1 (MQ=255)                                    ctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcg         <  1:359207/49‑1 (MQ=255)                                    ctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcg         <  1:1206020/49‑1 (MQ=255)                                     tcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc        >  1:566155/1‑49 (MQ=255)                                       aCTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc        >  1:2249224/1‑47 (MQ=255)                                       aCTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc        >  1:1826418/1‑47 (MQ=255)                                           tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATg  <  1:138433/49‑1 (MQ=255)                                           tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATg  <  1:1197311/49‑1 (MQ=255)                                                |                                            TTTTCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATG  >  NC_000913/1154315‑1154405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑