Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,240,439	C→T	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM → cvrA ←	protein YmgM putative K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,240,439	0	C	T	100.0%	45.3 / NA	14	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM cvrA	protein YmgM putative K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/6);  total (8/6)

CTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACC  >  NC_000913/1240394‑1240484                                              |                                              ctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTAcc                                            >  1:2088895/1‑49 (MQ=255)  tttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGa                                          >  1:1148630/1‑50 (MQ=255)           cACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTg                                 <  1:704476/50‑1 (MQ=255)                 ccAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCgcgc                           >  1:2404699/1‑50 (MQ=255)                  cAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGcc                          <  1:109697/50‑1 (MQ=255)                   aGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCt                         <  1:415832/50‑1 (MQ=255)                     atcatcCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTaa                       >  1:703445/1‑50 (MQ=255)                               cAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTgg             <  1:626326/50‑1 (MQ=255)                                aaaCTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGa            >  1:1907601/1‑50 (MQ=255)                                aaaCTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGa            <  1:205763/50‑1 (MQ=255)                                       aCCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAAttt      >  1:1052169/1‑49 (MQ=255)                                       aCCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAAttt      >  1:110023/1‑49 (MQ=255)                                         cTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCAc   <  1:2416605/50‑1 (MQ=255)                                          tGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCAcc  >  1:1589864/1‑50 (MQ=255)                                              |                                              CTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACC  >  NC_000913/1240394‑1240484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑