Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	80.6 / NA	23	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/17);  total (6/17)

TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTG  >  NC_000913/1382508‑1382600                                                |                                              ttGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             >  1:2011162/1‑50 (MQ=255)   gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGc                                            <  1:146292/49‑1 (MQ=255)    cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                          >  1:972286/1‑50 (MQ=255)          aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                     <  1:965771/49‑1 (MQ=255)          aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                     <  1:2551772/49‑1 (MQ=255)            cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTg                                  <  1:1399205/50‑1 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                               >  1:2037764/1‑50 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                          <  1:2433702/48‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                        <  1:1152499/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                  <  1:1993009/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                  <  1:204062/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                  <  1:675143/50‑1 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                >  1:1243039/1‑50 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt              <  1:2386548/49‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt              <  1:837173/49‑1 (MQ=255)                                  aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg            <  1:858560/50‑1 (MQ=255)                                   atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc           <  1:2406641/50‑1 (MQ=255)                                     atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc         >  1:2173349/1‑50 (MQ=255)                                         acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt     <  1:420706/50‑1 (MQ=255)                                          cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt    <  1:2427883/50‑1 (MQ=255)                                          cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt    <  1:1298825/50‑1 (MQ=255)                                           gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt   >  1:1964765/1‑50 (MQ=255)                                            acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg  <  1:2376732/50‑1 (MQ=255)                                                |                                              TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTG  >  NC_000913/1382508‑1382600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑