Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,028	T→C	V99A (GTC→GCC)	ampD →	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,028	0	T	C	100.0%	71.5 / NA	21	V99A (GTC→GCC)	ampD	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/8);  total (13/8)

AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATT  >  NC_000913/118981‑119071                                                |                                            aaTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcct                                           >  1:135274/1‑50 (MQ=255) aaTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcct                                           >  1:1468735/1‑50 (MQ=255)  aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc                                          >  1:366208/1‑50 (MQ=255)      tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                      >  1:909455/1‑50 (MQ=255)         aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                   <  1:837876/50‑1 (MQ=255)         aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                   >  1:724949/1‑50 (MQ=255)           tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                   >  1:2448133/1‑48 (MQ=255)              gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                              >  1:2514736/1‑50 (MQ=255)                    ttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg                         >  1:2085715/1‑49 (MQ=255)                     tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGaa                       >  1:489017/1‑50 (MQ=255)                      cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                      >  1:973986/1‑50 (MQ=255)                      cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                      >  1:159178/1‑50 (MQ=255)                       gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                     >  1:1837385/1‑50 (MQ=255)                         tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt                   <  1:2410388/50‑1 (MQ=255)                          aaaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                  >  1:852994/1‑50 (MQ=255)                           aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                  <  1:2205825/49‑1 (MQ=255)                             cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa               >  1:1864703/1‑50 (MQ=255)                               tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg             <  1:2193934/50‑1 (MQ=255)                                 catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt           >  1:1579011/1‑50 (MQ=255)                                     gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtttt        <  1:1795243/49‑1 (MQ=255)                                      catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc      <  1:1054010/50‑1 (MQ=255)                                       atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc      >  1:1821778/1‑49 (MQ=255)                                        tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTa    <  1:2502877/50‑1 (MQ=255)                                          cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAtt  <  1:2324188/50‑1 (MQ=255)                                                |                                            AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATT  >  NC_000913/118981‑119071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑