Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,662,872	C→T	R773W (CGG→TGG)	ynfF →	putative selenate reductase YnfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,662,872	0	C	T	100.0%	72.1 / NA	21	R773W (CGG→TGG)	ynfF	putative selenate reductase YnfF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/10);  total (11/10)

CCGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTC  >  NC_000913/1662825‑1662911                                                |                                        ccGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTgg                                       <  1:1776921/50‑1 (MQ=255)    ccATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTc                                    <  1:1627856/50‑1 (MQ=255)         gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCa                               >  1:1682338/1‑50 (MQ=255)           gCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcg                             >  1:854416/1‑50 (MQ=255)               ggTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggcgg                         >  1:2231321/1‑50 (MQ=255)                gTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggcgg                         >  1:1839042/1‑49 (MQ=255)                gTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACggcggc                        <  1:1276609/50‑1 (MQ=255)                gTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACggcggc                        <  1:2191355/50‑1 (MQ=255)                     tGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTAt                   <  1:1178358/50‑1 (MQ=255)                       gCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCa                 <  1:1040445/50‑1 (MQ=255)                          tAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAata              >  1:83353/1‑50 (MQ=255)                           aaaGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAatat             >  1:1448101/1‑50 (MQ=255)                           aaaGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAatat             >  1:371257/1‑50 (MQ=255)                           aaaGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAatat             >  1:938239/1‑50 (MQ=255)                            aaGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATAtt            >  1:120578/1‑50 (MQ=255)                               cGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTg         <  1:2539509/50‑1 (MQ=255)                                 gATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGAc       <  1:2554714/50‑1 (MQ=255)                                 gATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGAc       >  1:478971/1‑50 (MQ=255)                                     tGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACctct   <  1:546754/50‑1 (MQ=255)                                      gTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACctct   >  1:81887/1‑49 (MQ=255)                                       tttGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCtctc  <  1:382658/49‑1 (MQ=255)                                                |                                        CCGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTC  >  NC_000913/1662825‑1662911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑