Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,741,062	T→C	C384C (TGT→TGC)	ydhC →	putative transporter YdhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,741,062	0	T	C	100.0%	63.2 / NA	18	C384C (TGT→TGC)	ydhC	putative transporter YdhC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/9);  total (9/9)

AACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCA  >  NC_000913/1741020‑1741106                                           |                                             aaCAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCgaagaa                                        <  1:1259608/49‑1 (MQ=255)    agtagtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTg                                    >  1:900002/1‑50 (MQ=255)       agtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCt                                 >  1:1249864/1‑50 (MQ=255)       agtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCt                                 >  1:364656/1‑50 (MQ=255)        gtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCt                                 <  1:1503840/49‑1 (MQ=255)           cTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCa                             <  1:1686386/50‑1 (MQ=255)              gTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAg                            <  1:1490988/48‑1 (MQ=255)              gTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGaa                          >  1:130865/1‑50 (MQ=255)              gTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGaa                          >  1:1090187/1‑50 (MQ=255)                 gcgcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATc                        <  1:694883/49‑1 (MQ=255)                     tGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATgg                    >  1:2104716/1‑49 (MQ=255)                        gTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAAt                <  1:2463100/50‑1 (MQ=255)                         ttACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATg               >  1:32727/1‑50 (MQ=255)                           aCATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGc              <  1:2578710/49‑1 (MQ=255)                            cATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCg            <  1:201362/50‑1 (MQ=255)                               gatgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAg         >  1:321532/1‑50 (MQ=255)                                 tgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTc       <  1:216506/50‑1 (MQ=255)                                       gTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCa  >  1:822812/1‑49 (MQ=255)                                           |                                             AACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCA  >  NC_000913/1741020‑1741106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑