Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	86.0 / NA	24	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/10);  total (14/10)

GCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCC  >  NC_000913/1742710‑1742795                                              |                                         gcgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                      >  1:891355/1‑50 (MQ=255)  cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                     <  1:2617343/50‑1 (MQ=255)  cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                     <  1:2366230/50‑1 (MQ=255)       aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                <  1:404918/50‑1 (MQ=255)       aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                <  1:1699487/50‑1 (MQ=255)        gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATgg                               <  1:1999577/50‑1 (MQ=255)               cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                         >  1:518596/1‑49 (MQ=255)                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                      <  1:2323128/49‑1 (MQ=255)                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                        <  1:1096622/47‑1 (MQ=255)                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                     >  1:328223/1‑50 (MQ=255)                  cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                     >  1:442400/1‑50 (MQ=255)                   ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                    >  1:2122329/1‑50 (MQ=255)                   ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                    <  1:459000/50‑1 (MQ=255)                   ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                    >  1:744603/1‑50 (MQ=255)                    cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                   >  1:1586124/1‑50 (MQ=255)                      agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                 >  1:109693/1‑50 (MQ=255)                           cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg            >  1:425039/1‑50 (MQ=255)                            gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg            <  1:2011608/49‑1 (MQ=255)                                gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt       >  1:244723/1‑50 (MQ=255)                                gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt       >  1:275709/1‑50 (MQ=255)                                 ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt      <  1:1083550/50‑1 (MQ=255)                                   ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg    >  1:2465020/1‑50 (MQ=255)                                    cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc   >  1:1813856/1‑50 (MQ=255)                                     aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc  >  1:1350081/1‑50 (MQ=255)                                              |                                         GCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCC  >  NC_000913/1742710‑1742795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑