Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,533	G→A	G316D (GGC→GAC)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,533	0	G	A	100.0%	119.9 / NA	34	G316D (GGC→GAC)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/27);  total (7/27)

TAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTC  >  NC_000913/1776486‑1776574                                                |                                          tAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGAcc                                         >  1:18280/1‑50 (MQ=255)  aaaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCt                                        >  1:742149/1‑50 (MQ=255)    aGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTgg                                      <  1:767217/50‑1 (MQ=255)    aGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTgg                                      >  1:1671391/1‑50 (MQ=255)      gcgcGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTc                                    <  1:1211873/50‑1 (MQ=255)      gcgcGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTc                                    <  1:972258/50‑1 (MQ=255)           gTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCg                               <  1:1417625/50‑1 (MQ=255)            tGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCga                              <  1:2371223/50‑1 (MQ=255)            tGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCga                              <  1:697132/50‑1 (MQ=255)              gggAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCgaga                            <  1:237219/50‑1 (MQ=255)                gAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCgag                             >  1:185446/1‑47 (MQ=255)                  aTGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAgaagaa                        <  1:1542676/50‑1 (MQ=255)                   tGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGaagaag                       <  1:1164463/50‑1 (MQ=255)                     tCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGt                     <  1:475477/50‑1 (MQ=255)                       ggCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGtt                    <  1:2651083/49‑1 (MQ=255)                       ggCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGtt                    <  1:395797/49‑1 (MQ=255)                       ggCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGtt                    <  1:1597199/49‑1 (MQ=255)                        gCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgt                  <  1:1783374/50‑1 (MQ=255)                         cGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgtg                 <  1:1814694/50‑1 (MQ=255)                         cGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgtg                 <  1:2390149/50‑1 (MQ=255)                          gTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGc                <  1:1327105/50‑1 (MQ=255)                             ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtga             >  1:117420/1‑50 (MQ=255)                             ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtg              <  1:2440454/49‑1 (MQ=255)                             ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtg              <  1:1573367/49‑1 (MQ=255)                               tATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgatg           <  1:2680568/50‑1 (MQ=255)                                 ttGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGAc         <  1:141985/50‑1 (MQ=255)                                  tGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACt        >  1:2275295/1‑50 (MQ=255)                                   gCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACt        <  1:1447708/49‑1 (MQ=255)                                    cTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACtt       <  1:87075/49‑1 (MQ=255)                                    cTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACtt       <  1:881062/49‑1 (MQ=255)                                     tGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTa     >  1:2473077/1‑50 (MQ=255)                                       aCGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTa     <  1:253236/48‑1 (MQ=255)                                       aCGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTAtt   <  1:2101711/50‑1 (MQ=255)                                        cGGCATTGACCTGGTCGCCCGAGAAGAATGTTGTGCTGATGACTTTATTc  <  1:2298777/50‑1 (MQ=39)                                                |                                          TAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTC  >  NC_000913/1776486‑1776574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑