Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,208	T→C	V92A (GTT→GCT)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,208	0	T	C	100.0%	114.7 / NA	32	V92A (GTT→GCT)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/17);  total (15/17)

CAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTT  >  NC_000913/1811162‑1811251                                               |                                            cAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGc                                          >  1:256791/1‑50 (MQ=255)       aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                    <  1:913223/50‑1 (MQ=255)        tCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                   >  1:426305/1‑50 (MQ=255)        tCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                   <  1:319915/50‑1 (MQ=255)          gTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCa                                 <  1:699191/50‑1 (MQ=255)           ttATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCa                                 >  1:1859349/1‑49 (MQ=255)           ttATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                <  1:1134247/50‑1 (MQ=255)            tATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                >  1:867274/1‑49 (MQ=255)             aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATaa                              >  1:1850117/1‑50 (MQ=255)             aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATaa                              >  1:2327038/1‑50 (MQ=255)              tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                             <  1:1520999/50‑1 (MQ=255)              tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                             <  1:2037746/50‑1 (MQ=255)               gCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                            >  1:779266/1‑50 (MQ=255)               gCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                            >  1:1495647/1‑50 (MQ=255)                ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                            <  1:1485369/49‑1 (MQ=255)                      gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                      <  1:1542300/49‑1 (MQ=255)                      gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                      <  1:2594565/49‑1 (MQ=255)                         ttCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                   >  1:940665/1‑49 (MQ=255)                          tCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                   >  1:525681/1‑48 (MQ=255)                           cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGtt                  <  1:80996/48‑1 (MQ=255)                           cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTa                 <  1:2096376/49‑1 (MQ=255)                            gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAg                <  1:962213/49‑1 (MQ=255)                             ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGc              <  1:680829/50‑1 (MQ=255)                                ctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa            >  1:281975/1‑49 (MQ=255)                                   ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa          <  1:92421/48‑1 (MQ=255)                                   ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt         <  1:753155/49‑1 (MQ=255)                                   ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt         <  1:2666610/49‑1 (MQ=255)                                   ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt         >  1:1558250/1‑49 (MQ=255)                                    tCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt         >  1:2645041/1‑48 (MQ=255)                                    tCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt         >  1:2169311/1‑48 (MQ=255)                                     cTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCa       <  1:2030771/49‑1 (MQ=255)                                         gcgcGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtttt  >  1:872076/1‑50 (MQ=255)                                               |                                            CAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTT  >  NC_000913/1811162‑1811251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑