Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,898,152	G→A	A407A (GCG→GCA)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,898,152	0	G	A	100.0%	85.8 / NA	24	A407A (GCG→GCA)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/10);  total (14/10)

CATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATA  >  NC_000913/1898107‑1898198                                              |                                               cATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACgg                                             <  1:178177/49‑1 (MQ=255)  aTTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGAt                                           <  1:1517217/50‑1 (MQ=255)     gAGCGTAATACCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGc                                        >  1:2328719/1‑50 (MQ=38)      aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGct                                       >  1:456173/1‑50 (MQ=255)      aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGc                                        <  1:1173798/49‑1 (MQ=255)      aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGc                                        <  1:563032/49‑1 (MQ=255)       gCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctc                                      >  1:906415/1‑50 (MQ=255)       gCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctc                                      <  1:455577/50‑1 (MQ=255)         gTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTg                                    >  1:1906070/1‑50 (MQ=255)             tGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGcgcc                                >  1:416635/1‑50 (MQ=255)               ccATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGcgccg                               >  1:17691/1‑49 (MQ=255)                  ttGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCAc                            >  1:222604/1‑49 (MQ=255)                    gCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCa                          <  1:2317078/49‑1 (MQ=255)                    gCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAg                         >  1:888676/1‑50 (MQ=255)                     ccTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAg                         <  1:1630519/49‑1 (MQ=255)                     ccTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGt                        <  1:728051/50‑1 (MQ=255)                      ctctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTg                       >  1:2318412/1‑50 (MQ=255)                      ctctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTg                       >  1:1861350/1‑50 (MQ=255)                        ctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCa                     >  1:1019028/1‑50 (MQ=255)                           tgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACcg                  <  1:2120810/50‑1 (MQ=255)                                gCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGt             >  1:419939/1‑50 (MQ=255)                                  gATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtct           <  1:679015/50‑1 (MQ=255)                                      aaCGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCt       >  1:2575163/1‑50 (MQ=255)                                        cgccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTg      >  1:1254633/1‑49 (MQ=255)                                           cgcacgGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGAt   >  1:2470636/1‑49 (MQ=255)                                           cgcacgGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATa  <  1:460286/50‑1 (MQ=255)                                              |                                               CATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATA  >  NC_000913/1898107‑1898198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑