Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,784	A→G	V267A (GTC→GCC)	znuA ←	Zn(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,784	0	A	G	100.0%	87.4 / NA	25	V267A (GTC→GCC)	znuA	Zn(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/11);  total (14/11)

CCCAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGC  >  NC_000913/1941739‑1941829                                              |                                              cccAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGct                                           >  1:2341203/1‑50 (MQ=255)     ggggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCa                                       <  1:84574/50‑1 (MQ=255)     ggggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCa                                       <  1:2570674/50‑1 (MQ=255)     ggggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCa                                       <  1:2193483/50‑1 (MQ=255)      gggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCaa                                      <  1:2086745/50‑1 (MQ=255)           ccAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGAcc                                 >  1:2636157/1‑50 (MQ=255)              aCGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCt                              <  1:283646/50‑1 (MQ=255)                  tCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGcc                          >  1:2204549/1‑50 (MQ=255)                    ccATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCt                         >  1:1282974/1‑49 (MQ=255)                    ccATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCt                         <  1:522484/49‑1 (MQ=255)                      aTACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGaa                      >  1:1938627/1‑50 (MQ=255)                       tACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAAc                     >  1:1103958/1‑50 (MQ=255)                          gAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACtgt                  >  1:1535060/1‑50 (MQ=255)                           aaCGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACtgtg                 >  1:897077/1‑50 (MQ=255)                            aCGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTgg                <  1:212151/50‑1 (MQ=255)                             cGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGc               <  1:387741/50‑1 (MQ=255)                             cGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGc               >  1:1124712/1‑50 (MQ=255)                              ggATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt              >  1:910205/1‑50 (MQ=255)                              ggATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt              >  1:2176046/1‑50 (MQ=255)                              ggATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt              >  1:17581/1‑50 (MQ=255)                                aTGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGTACTGTGGCt              >  1:589539/1‑48 (MQ=255)                                 tGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAg           <  1:1930469/50‑1 (MQ=255)                                  gTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAg           >  1:2237749/1‑49 (MQ=255)                                   tCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGc          <  1:732535/49‑1 (MQ=255)                                          tGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGc  <  1:1347585/50‑1 (MQ=255)                                           gcggcgCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACg   >  1:2435764/1‑48 (MQ=255)                                              |                                              CCCAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACGC  >  NC_000913/1941739‑1941829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑