| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,040,801 | T→C | L107L (TTA→CTA) | msrQ → | periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,040,801 | 0 | T | C | 95.8% | 80.9 / ‑4.2 | 24 | L107L (TTA→CTA) | msrQ | periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (10/13); minor base A (0/1); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
TTTAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGTTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAGGTAT > NC_000913/2040755‑2040847 | tttAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGctat > 1:1733396/1‑50 (MQ=255) ttAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGctat > 1:260328/1‑49 (MQ=255) tAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGctatt < 1:946665/49‑1 (MQ=255) aCAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAg < 1:2572357/49‑1 (MQ=255) cAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGt < 1:2622660/50‑1 (MQ=255) aaGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGATATTAGGTa < 1:67097/50‑1 (MQ=255) aGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGt > 1:756699/1‑48 (MQ=255) aGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGt > 1:2069704/1‑48 (MQ=255) gTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAg < 1:2384277/50‑1 (MQ=255) gCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGtt < 1:29013/50‑1 (MQ=255) tGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAAt > 1:873927/1‑49 (MQ=255) gCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAAtt < 1:623412/49‑1 (MQ=255) gCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAAtt < 1:2453790/49‑1 (MQ=255) cTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTAc > 1:2092535/1‑50 (MQ=255) gAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCg < 1:256324/50‑1 (MQ=255) tGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCt > 1:1160462/1‑50 (MQ=255) gggCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCt > 1:897938/1‑49 (MQ=255) ggCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCtt < 1:755486/49‑1 (MQ=255) ggCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTa < 1:1541303/50‑1 (MQ=255) tGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTa < 1:240357/50‑1 (MQ=255) tGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTa < 1:519422/50‑1 (MQ=255) aataatCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAAc < 1:1039741/50‑1 (MQ=255) aatCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGt > 1:795654/1‑49 (MQ=255) atCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGt > 1:2079580/1‑48 (MQ=255) atCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTa < 1:2255508/50‑1 (MQ=255) tGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAgg > 1:1813783/1‑49 (MQ=255) tGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAgg > 1:1803572/1‑49 (MQ=255) gcgcTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAg < 1:732249/46‑1 (MQ=255) gcgcTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAGGtat < 1:2475392/50‑1 (MQ=255) | TTTAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGTTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAGGTAT > NC_000913/2040755‑2040847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |