Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,885	C→T	T18I (ACC→ATC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,885	0	C	T	100.0%	100.7 / NA	28	T18I (ACC→ATC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/10);  total (18/10)

TGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGA  >  NC_000913/214840‑214929                                              |                                             tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                           >  1:2243455/1‑49 (MQ=255)      tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                      <  1:561459/49‑1 (MQ=255)       cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                     <  1:2559088/49‑1 (MQ=255)         cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                  >  1:69381/1‑50 (MQ=255)           aCATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcgc                                <  1:70256/50‑1 (MQ=255)            cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                 >  1:1164297/1‑48 (MQ=255)            cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                 >  1:2177194/1‑48 (MQ=255)             aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                              <  1:1371203/50‑1 (MQ=255)                ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAggg                            <  1:1944723/49‑1 (MQ=255)                   cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                        >  1:393310/1‑50 (MQ=255)                   cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                        >  1:2625156/1‑50 (MQ=255)                     aTTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAAGGCgcggg                      >  1:1752959/1‑50 (MQ=38)                        cccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                   >  1:2701011/1‑50 (MQ=255)                         ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                   <  1:2619769/49‑1 (MQ=255)                            aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag               >  1:2046895/1‑50 (MQ=255)                             tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag               >  1:1643373/1‑49 (MQ=255)                             tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag               >  1:736002/1‑49 (MQ=255)                                 gAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt          >  1:2324237/1‑50 (MQ=255)                                 gAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt          >  1:2122527/1‑50 (MQ=255)                                 gAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt          >  1:2063793/1‑50 (MQ=255)                                 gAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt          >  1:2016622/1‑50 (MQ=255)                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt         <  1:1009354/50‑1 (MQ=255)                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt          >  1:2372385/1‑49 (MQ=255)                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt          >  1:222600/1‑49 (MQ=255)                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt          <  1:465300/49‑1 (MQ=255)                                   gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta        <  1:161842/50‑1 (MQ=255)                                       gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat      >  1:508385/1‑48 (MQ=255)                                         gaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGa  <  1:1935387/50‑1 (MQ=255)                                              |                                             TGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGA  >  NC_000913/214840‑214929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑