Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,004	T→C	E43G (GAA→GGA)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,004	0	T	C	100.0%	83.3 / NA	23	E43G (GAA→GGA)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/9);  total (14/9)

TGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAA  >  NC_000913/2661963‑2662050                                          |                                               tGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                          >  1:1701186/1‑48 (MQ=255)  ggAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                          >  1:599173/1‑47 (MQ=255)  ggAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                          >  1:1915282/1‑47 (MQ=255)   gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATaa                                        <  1:88822/48‑1 (MQ=255)   gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAg                                       <  1:118127/49‑1 (MQ=255)      aCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAg                                   <  1:779041/50‑1 (MQ=255)        cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg                                  >  1:1107810/1‑49 (MQ=255)        cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg                                  >  1:1508993/1‑49 (MQ=255)          gACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGaa                               <  1:343763/50‑1 (MQ=255)           aCCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGaaa                              >  1:2515059/1‑50 (MQ=255)             cATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt                            >  1:1667596/1‑50 (MQ=255)                ttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc                         >  1:912464/1‑50 (MQ=255)                ttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc                         >  1:1031271/1‑50 (MQ=255)                ttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc                         >  1:1628126/1‑50 (MQ=255)                 tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt                        >  1:2506327/1‑50 (MQ=255)                  ttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt                        >  1:228486/1‑49 (MQ=255)                   tACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTg                       <  1:2550006/49‑1 (MQ=255)                     cGCAGACGGGAAAACAGTTTTCCCAGATAAGAAAGGGAAATTCCCTGAc                     <  1:2265663/49‑1 (MQ=39)                     cGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGAc                     <  1:133106/49‑1 (MQ=255)                         gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg                <  1:1335408/50‑1 (MQ=255)                            gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa              >  1:350559/1‑49 (MQ=255)                                aaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat         >  1:2343484/1‑50 (MQ=255)                                aaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat         <  1:2582754/50‑1 (MQ=255)                                       tgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCaa  >  1:1642891/1‑50 (MQ=255)                                          |                                               TGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAA  >  NC_000913/2661963‑2662050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑