Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,793,867	C→A	A378D (GCT→GAT)	gabT →	4‑aminobutyrate aminotransferase GabT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,793,867	0	C	A	100.0%	93.1 / NA	26	A378D (GCT→GAT)	gabT	4‑aminobutyrate aminotransferase GabT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/9);  total (17/9)

CGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTA  >  NC_000913/2793820‑2793912                                                |                                              cGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATc                                             >  1:2163122/1‑50 (MQ=255)  ggCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATcg                                            <  1:536993/50‑1 (MQ=255)  ggCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATcg                                            >  1:2687327/1‑50 (MQ=255)  ggCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATcg                                            >  1:1376375/1‑50 (MQ=255)       tCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCGCGccc                                       >  1:421726/1‑50 (MQ=255)       tCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCGCGccc                                       <  1:2484314/50‑1 (MQ=255)             caaGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATa                                 <  1:1482597/50‑1 (MQ=255)               aGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATa                                 >  1:1089025/1‑48 (MQ=255)                   ggACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGcc                           <  1:688126/50‑1 (MQ=255)                   ggACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGcc                           >  1:2679938/1‑50 (MQ=255)                    gACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCt                          >  1:2480268/1‑50 (MQ=255)                      cGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGa                        >  1:2563550/1‑50 (MQ=255)                        ccAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGAtt                      <  1:2013941/50‑1 (MQ=255)                          aaaCTCACCGCCGAGATCTTGGATCGCGCCCGCGATAAAGGCCTGAttct                    >  1:927452/1‑50 (MQ=255)                            aCTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGAttcttc                  >  1:1952420/1‑50 (MQ=255)                            aCTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGAttcttc                  >  1:1104395/1‑50 (MQ=255)                             cTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATtcttct                 >  1:1799258/1‑50 (MQ=255)                               cACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTtctct               >  1:1404891/1‑50 (MQ=255)                                   gccgAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTg           <  1:525064/50‑1 (MQ=255)                                      gagaTCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCgg        >  1:2499675/1‑50 (MQ=255)                                        gaTCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGcc      >  1:606538/1‑50 (MQ=255)                                        gaTCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGcc      >  1:724823/1‑50 (MQ=255)                                         aTCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGccc     >  1:1083041/1‑50 (MQ=255)                                          tCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCg    <  1:282270/50‑1 (MQ=255)                                            gTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGt   <  1:1168887/49‑1 (MQ=255)                                            gTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTa  <  1:1618642/50‑1 (MQ=255)                                                |                                              CGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTA  >  NC_000913/2793820‑2793912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑