Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,364	T→C	V250A (GTC→GCC)	emrB →	multidrug efflux pump membrane subunit EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,364	0	T	C	100.0%	96.6 / NA	27	V250A (GTC→GCC)	emrB	multidrug efflux pump membrane subunit EmrB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/12);  total (15/12)

TATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTT  >  NC_000913/2813317‑2813409                                                |                                              tATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCt                                             >  1:2561675/1‑50 (MQ=255)    catcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTgg                                           >  1:202223/1‑49 (MQ=255)     atcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTgg                                           >  1:1131769/1‑48 (MQ=255)        cTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCt                                      >  1:1225968/1‑50 (MQ=255)        cTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCt                                      <  1:1574658/50‑1 (MQ=255)             cGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGAcc                                  >  1:1691990/1‑49 (MQ=255)               tggtggCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACcgac                               >  1:2125503/1‑50 (MQ=255)                ggtggCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACcgac                               >  1:1397748/1‑49 (MQ=255)                   ggCGGTGGTGGCTATCTGCTCCCTGATTGCCTGGGAGCTGACCGACGAt                            >  1:591505/1‑49 (MQ=39)                    gCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATa                           <  1:2077016/49‑1 (MQ=255)                       gtggtgGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAccc                       <  1:1306730/50‑1 (MQ=255)                       gtggtgGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAccc                       <  1:2106656/50‑1 (MQ=255)                       gtggtgGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAccc                       <  1:933296/50‑1 (MQ=255)                        tggtggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCg                      <  1:932939/50‑1 (MQ=255)                          gtggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGa                     >  1:1075978/1‑49 (MQ=255)                          gtggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGa                     >  1:550418/1‑49 (MQ=255)                          gtggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGa                     <  1:21688/49‑1 (MQ=255)                             gCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGt                 <  1:2514088/50‑1 (MQ=255)                                 tCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCg               <  1:800981/48‑1 (MQ=255)                                    gCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTg          >  1:1060682/1‑50 (MQ=255)                                     cTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCt           <  1:2039129/48‑1 (MQ=255)                                     cTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTg          <  1:2381395/49‑1 (MQ=255)                                     cTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTg          <  1:1653807/49‑1 (MQ=255)                                       tCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCg       >  1:151054/1‑50 (MQ=255)                                        ccTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCgt      >  1:581413/1‑50 (MQ=255)                                            aTTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCgttgtt  >  1:1976212/1‑50 (MQ=255)                                            aTTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCgttgtt  >  1:1734679/1‑50 (MQ=255)                                                |                                              TATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTT  >  NC_000913/2813317‑2813409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑