Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,090,282	C→T	intergenic (+30/‑65)	yggI → / → endA	conserved protein YggI/DNA‑specific endonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,090,282	0	C	T	100.0%	89.7 / NA	26	intergenic (+30/‑65)	yggI/endA	conserved protein YggI/DNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/8);  total (18/8)

CAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGT  >  NC_000913/3090235‑3090328                                                |                                               cAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTg                                              >  1:1469788/1‑50 (MQ=255)    cTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCt                                           <  1:595067/50‑1 (MQ=255)      ggTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGa                                         >  1:1106343/1‑50 (MQ=255)          gCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTg                                      <  1:357296/49‑1 (MQ=255)          gCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTg                                      >  1:1523308/1‑49 (MQ=255)            gAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCa                                    >  1:866373/1‑49 (MQ=255)             aaaTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATa                                  >  1:1665099/1‑50 (MQ=255)               aTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAc                                 >  1:1162510/1‑49 (MQ=255)                  aCCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaa                              >  1:1305110/1‑49 (MQ=255)                  aCCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaa                              >  1:1556967/1‑49 (MQ=255)                   ccATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                            >  1:2468783/1‑50 (MQ=255)                    cATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAAc                           <  1:2406985/50‑1 (MQ=255)                     aTCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                            >  1:1055380/1‑48 (MQ=255)                     aTCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                            >  1:2184297/1‑48 (MQ=255)                         gAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCtt                      >  1:1320883/1‑50 (MQ=255)                          aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCttt                     <  1:1535899/50‑1 (MQ=255)                                 aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTAc               <  1:254352/49‑1 (MQ=255)                                 aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACg              >  1:2022733/1‑50 (MQ=255)                                 aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACg              <  1:2654696/50‑1 (MQ=255)                                   gAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGtt            <  1:614594/50‑1 (MQ=255)                                        ttCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTgg       >  1:152947/1‑50 (MQ=255)                                         tCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGc      >  1:844202/1‑50 (MQ=255)                                          ccTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGc      <  1:1434041/49‑1 (MQ=255)                                          ccTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCt     >  1:223238/1‑50 (MQ=255)                                            tGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCg   >  1:1015209/1‑50 (MQ=255)                                             gATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGt  >  1:1731010/1‑50 (MQ=255)                                                |                                               CAGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGT  >  NC_000913/3090235‑3090328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑