Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,527,760	A→G	N431S (AAC→AGC)	igaA →	inner membrane protein ‑ inhibits the Rcs signaling pathway

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,527,760	0	A	G	100.0%	89.7 / NA	25	N431S (AAC→AGC)	igaA	inner membrane protein ‑ inhibits the Rcs signaling pathway
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/10);  total (15/10)

ACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAAC  >  NC_000913/3527714‑3527803                                               |                                            aCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcga                                          >  1:1454737/1‑50 (MQ=255)   cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg                                        >  1:1186941/1‑50 (MQ=255)     aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc                                      >  1:556099/1‑50 (MQ=255)        cACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCg                                    <  1:2478614/49‑1 (MQ=255)        cACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGc                                   >  1:670360/1‑50 (MQ=255)        cACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGc                                   >  1:1451309/1‑50 (MQ=255)         aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt                                  <  1:757981/50‑1 (MQ=255)          ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTc                                 <  1:1663218/50‑1 (MQ=255)           cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa                                <  1:1753675/50‑1 (MQ=255)              ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt                              <  1:1509399/49‑1 (MQ=255)              ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg                             >  1:659658/1‑50 (MQ=255)                  aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt                         >  1:2309263/1‑50 (MQ=255)                   ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAtt                        <  1:2609553/50‑1 (MQ=255)                    cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa                       >  1:2674400/1‑50 (MQ=255)                       ttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc                      >  1:1128520/1‑48 (MQ=255)                        tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCa                    <  1:1775668/49‑1 (MQ=255)                         gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg                   >  1:232306/1‑49 (MQ=255)                         gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg                   >  1:1418109/1‑49 (MQ=255)                         gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg                   <  1:566366/49‑1 (MQ=255)                         gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg                   >  1:1346566/1‑49 (MQ=255)                         gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg                   >  1:1668473/1‑49 (MQ=255)                                 gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGaa           <  1:339286/49‑1 (MQ=255)                                  cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt         <  1:2573400/50‑1 (MQ=255)                                   aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt         >  1:725132/1‑49 (MQ=255)                                       catcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc     >  1:222807/1‑49 (MQ=255)                                        atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa   >  1:1894688/1‑50 (MQ=255)                                          cTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaac  >  1:2698349/1‑49 (MQ=255)                                               |                                            ACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAAC  >  NC_000913/3527714‑3527803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑