Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,829,233	C→A	L97M (CTG→ATG)	xanP →	xanthine:H(+) symporter XanP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,829,233	0	C	A	100.0%	88.4 / NA	25	L97M (CTG→ATG)	xanP	xanthine:H(+) symporter XanP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/13);  total (12/13)

ATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGCTGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGA  >  NC_000913/3829190‑3829278                                            |                                              aTCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGAtgttgt                                         <  1:1557214/50‑1 (MQ=255)    gATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTa                                      >  1:1023101/1‑50 (MQ=255)    gATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTa                                      <  1:257458/50‑1 (MQ=255)    gATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTa                                      >  1:2523784/1‑50 (MQ=255)     attattCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTAt                                     >  1:911500/1‑50 (MQ=255)            aaaTTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAggg                               >  1:2177149/1‑49 (MQ=255)            aaaTTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGc                              <  1:1516085/50‑1 (MQ=255)             aaTTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCa                             >  1:2494045/1‑50 (MQ=255)              aTTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCAc                            >  1:702659/1‑50 (MQ=255)               ttAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCAc                            >  1:2021627/1‑49 (MQ=255)                 aaGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCa                          <  1:253197/49‑1 (MQ=255)                  aGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAg                         <  1:2533556/49‑1 (MQ=255)                     ccTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCtt                      >  1:746491/1‑49 (MQ=255)                      cTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTc                     >  1:274999/1‑49 (MQ=255)                              cGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTttgtt            <  1:2266738/50‑1 (MQ=255)                               ggTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTttgttg           >  1:2050034/1‑50 (MQ=255)                               ggTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTttgttg           <  1:891668/50‑1 (MQ=255)                                gTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGc          <  1:2104118/50‑1 (MQ=255)                                 ttGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGcc         <  1:1988386/50‑1 (MQ=255)                                   ggCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGcccc       >  1:829716/1‑50 (MQ=255)                                    gCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGcccc       <  1:1779490/49‑1 (MQ=255)                                    gCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCg      <  1:2123147/50‑1 (MQ=255)                                        cGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCt    <  1:1402605/48‑1 (MQ=255)                                        cGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCt    <  1:49855/48‑1 (MQ=255)                                        cGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGa  >  1:995058/1‑50 (MQ=255)                                            |                                              ATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGCTGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGA  >  NC_000913/3829190‑3829278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑