Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,850,891	G→A	Q68* (CAA→TAA)	ilvN ←	acetohydroxy acid synthase I subunit IlvN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,850,891	0	G	A	100.0%	119.9 / NA	34	Q68* (CAA→TAA)	ilvN	acetohydroxy acid synthase I subunit IlvN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

GGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAG  >  NC_000913/3850844‑3850937                                                |                                               ggaCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGc                                              <  1:1457589/50‑1 (MQ=255) ggaCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGc                                              <  1:182432/50‑1 (MQ=255) ggaCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGc                                              >  1:232623/1‑50 (MQ=255)    cTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTa                                           <  1:2689729/50‑1 (MQ=255)     tGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                          >  1:1124242/1‑50 (MQ=255)      gATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                          >  1:1088175/1‑49 (MQ=255)      gATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                          >  1:894888/1‑49 (MQ=255)       aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatca                                        >  1:455405/1‑50 (MQ=255)       aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatc                                         <  1:2515613/49‑1 (MQ=255)       aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                          <  1:2484704/48‑1 (MQ=255)        ttACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatcat                                       <  1:2458599/50‑1 (MQ=255)          aCGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatcatc                                      <  1:283305/49‑1 (MQ=255)           cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTg                                    <  1:2183164/50‑1 (MQ=255)            gCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGc                                   >  1:2373523/1‑50 (MQ=255)             cTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCt                                  >  1:2425485/1‑50 (MQ=255)               gCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTc                                 >  1:626740/1‑49 (MQ=255)                  cTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGa                             >  1:929962/1‑50 (MQ=255)                  cTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGa                             <  1:500453/50‑1 (MQ=255)                       aCGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCt                         <  1:523750/49‑1 (MQ=255)                         gACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgt                      <  1:1022176/50‑1 (MQ=255)                           cATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtcg                    >  1:2052631/1‑50 (MQ=255)                           cATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtcg                    <  1:1893046/50‑1 (MQ=255)                                 ccAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCAtt               >  1:1503664/1‑49 (MQ=255)                                  cAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGa             >  1:936418/1‑50 (MQ=255)                                    gCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc            >  1:1566592/1‑49 (MQ=255)                                    gCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc            >  1:225539/1‑49 (MQ=255)                                       tATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg         >  1:268602/1‑49 (MQ=255)                                       tATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg         >  1:650032/1‑49 (MQ=255)                                        aTCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGt        <  1:2266353/49‑1 (MQ=255)                                         tCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTa       <  1:2591204/49‑1 (MQ=255)                                         tCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAg      <  1:1356159/50‑1 (MQ=255)                                          cGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc     >  1:2523745/1‑50 (MQ=255)                                             tttAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAg  <  1:139835/50‑1 (MQ=255)                                             tttAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAg  <  1:1210521/50‑1 (MQ=255)                                                |                                               GGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAG  >  NC_000913/3850844‑3850937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑