Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,696	C→T	G103D (GGC→GAC)	asnC ←	DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,696	0	C	T	100.0%	96.6 / NA	28	G103D (GGC→GAC)	asnC	DNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/13);  total (15/13)

GAGCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTT  >  NC_000913/3926649‑3926742                                                |                                               gagCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCg                                              <  1:2515217/50‑1 (MQ=255)    cGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGtt                                           <  1:990093/50‑1 (MQ=255)    cGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGtt                                           <  1:1457896/50‑1 (MQ=255)    cGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGtt                                           <  1:551408/50‑1 (MQ=255)         atcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTa                                      <  1:1174335/50‑1 (MQ=255)            gaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATa                                   <  1:2411926/50‑1 (MQ=255)             aaCGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAg                                  >  1:172258/1‑50 (MQ=255)              aCGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAgg                                 >  1:2049314/1‑50 (MQ=255)                ggCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCt                               <  1:1600508/50‑1 (MQ=255)                  cacaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                              >  1:599760/1‑49 (MQ=255)                   acaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                              >  1:111911/1‑48 (MQ=255)                     aTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGt                          >  1:2424491/1‑50 (MQ=255)                      tCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTg                         <  1:2435891/50‑1 (MQ=255)                      tCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTg                         <  1:570831/50‑1 (MQ=255)                       cACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGa                        >  1:1936275/1‑50 (MQ=255)                        aCTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGAc                       >  1:94596/1‑50 (MQ=255)                         cTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACt                      >  1:985896/1‑50 (MQ=255)                          ttttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACtt                     >  1:541148/1‑50 (MQ=255)                            ttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACtt                     <  1:1392767/48‑1 (MQ=255)                            ttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtca                   >  1:836594/1‑50 (MQ=255)                               taAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatca                <  1:2700139/50‑1 (MQ=255)                               taAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc                 <  1:2537771/49‑1 (MQ=255)                                aaaGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc                 >  1:1860490/1‑48 (MQ=255)                                   gATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGc            >  1:2184259/1‑50 (MQ=255)                                    aTGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCt           >  1:273581/1‑50 (MQ=255)                                    aTGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCt           >  1:1673726/1‑50 (MQ=255)                                          tAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAg     >  1:1650886/1‑50 (MQ=255)                                             tGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCtt  <  1:1082037/50‑1 (MQ=255)                                                |                                               GAGCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTT  >  NC_000913/3926649‑3926742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑