Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	140.7 / NA	31	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (18/13);  total (18/13)

CCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAAC  >  NC_000913/765141‑765231                                                 |                                           ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                                           <  1:1943892/50‑1 (MQ=255)      tttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                     >  1:1257203/1‑50 (MQ=39)      tttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                     >  1:672669/1‑50 (MQ=39)         gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                  >  1:2265689/1‑50 (MQ=39)          tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                  <  1:658971/49‑1 (MQ=38)          tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                 >  1:1974727/1‑50 (MQ=39)           gCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                >  1:2206172/1‑50 (MQ=39)            cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                               >  1:1500248/1‑50 (MQ=39)              tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                             >  1:1500970/1‑50 (MQ=39)              tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                             >  1:503227/1‑50 (MQ=39)               aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                             <  1:1717624/49‑1 (MQ=38)               aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                            >  1:881790/1‑50 (MQ=39)               aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                            >  1:1467011/1‑50 (MQ=39)                tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                            >  1:416068/1‑49 (MQ=38)                  gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAt                         <  1:2019178‑M1/50‑2 (MQ=255)                    gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                       <  1:1524421‑M1/50‑4 (MQ=255)                    gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                       >  1:683154‑M1/1‑47 (MQ=255)                       gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                    >  1:568265‑M1/1‑44 (MQ=255)                        atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                   <  1:281803‑M1/50‑8 (MQ=255)                        atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                    <  1:2197666‑M1/49‑7 (MQ=255)                         ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                  <  1:2233006‑M1/50‑9 (MQ=255)                         ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                  <  1:276508‑M1/50‑9 (MQ=255)                               cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg            <  1:1518398‑M1/50‑15 (MQ=255)                                 gcgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg            >  1:2160099‑M1/1‑34 (MQ=255)                                  cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaa          <  1:1713473‑M1/49‑17 (MQ=255)                                    cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcc       <  1:414999‑M1/50‑20 (MQ=255)                                     tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca      >  1:1424060‑M1/1‑30 (MQ=255)                                       tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca    <  1:1876234‑M1/50‑23 (MQ=255)                                        cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca    >  1:1738469‑M1/1‑27 (MQ=255)                                        cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca    >  1:1534248‑M1/1‑27 (MQ=255)                                         aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata  >  1:554677‑M1/1‑26 (MQ=255)                                         aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacat   <  1:1186916‑M1/49‑24 (MQ=255)                                                 |                                           CCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAAC  >  NC_000913/765141‑765231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑