Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,984	(G)7→8	coding (1041/1245 nt)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,977	1	.	G	92.9%	43.9 / ‑1.9	14	coding (1034/1245 nt)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (0/13);  total (1/13)
Fisher's exact test for biased strand distribution p-value = 7.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGT  >  NC_000913/3240931‑3241018                                                |                                          ttGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg                                        <  1:626121/50‑1 (MQ=255) ttGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg                                        <  1:281444/50‑1 (MQ=255) ttGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggg                                        >  1:536977/1‑50 (MQ=255) ttGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g                                          <  1:33298/48‑1 (MQ=255)              cTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgct                            <  1:2074278/50‑1 (MQ=255)              cTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgct                            <  1:61674/50‑1 (MQ=255)                 ctctGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgctg                           <  1:2055870/48‑1 (MQ=39)                 ctctGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCCTctgctg                           <  1:832227/48‑1 (MQ=38)                      tgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGAt                      <  1:1626229/48‑1 (MQ=39)                      tgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATc                     <  1:2069388/49‑1 (MQ=39)                      tgtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATc                     <  1:280161/49‑1 (MQ=39)                        tgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATccc                   <  1:910333/49‑1 (MQ=39)                        tgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATccc                   <  1:1071133/49‑1 (MQ=39)                        tgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                  <  1:1744091/50‑1 (MQ=255)                           cTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTg               <  1:1931289/50‑1 (MQ=255)                                    aTCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAat      <  1:1186855/50‑1 (MQ=255)                                       cGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATg   <  1:417973/50‑1 (MQ=255)                                       cGGCGTGGC‑GGGGGGGGCTCTGCTGCTGATTCCCCTGGCCTGTAatattt  >  1:725861/1‑48 (MQ=255)                                                |                                          TTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGT  >  NC_000913/3240931‑3241018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑