Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,086	C→T	intergenic (‑300/‑77)	yaaI ← / → dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,086	0	C	T	100.0%	84.6 / NA	25	intergenic (‑300/‑77)	yaaI/dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

GTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACAT  >  NC_000913/12042‑12132                                             |                                               gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTg                                           <  1:97717/50‑1 (MQ=255)   agtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGa                                          >  1:65339/1‑49 (MQ=255)     tCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                       <  1:143783/50‑1 (MQ=255)     tCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                       <  1:1566725/50‑1 (MQ=255)         ccGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGa                                   >  1:1220335/1‑50 (MQ=255)         ccGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGa                                   <  1:730189/50‑1 (MQ=255)                  gtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGcc                          >  1:2199296/1‑50 (MQ=255)                   tgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGccc                         <  1:364146/50‑1 (MQ=255)                      gtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTa                      >  1:145869/1‑50 (MQ=255)                        cTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTAt                     >  1:1351279/1‑49 (MQ=255)                           cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTAc                  <  1:450274/49‑1 (MQ=255)                           cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTAc                  <  1:2138972/49‑1 (MQ=255)                           cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACa                 <  1:863300/50‑1 (MQ=255)                             aaaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGa               >  1:1818168/1‑50 (MQ=255)                                aaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACt             >  1:1207841/1‑49 (MQ=255)                                aaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTc            <  1:1288094/50‑1 (MQ=255)                                  aTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcac          <  1:1551580/50‑1 (MQ=255)                                  aTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTca           >  1:1969149/1‑49 (MQ=255)                                   tGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcaca         <  1:1985051/50‑1 (MQ=255)                                     aaaTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACaa        <  1:2250675/49‑1 (MQ=255)                                      aaTGGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCTCATcc      <  1:1500169/50‑1 (MQ=255)                                          gggTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACcaca   <  1:1179216/49‑1 (MQ=255)                                          gggTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACat  >  1:1043787/1‑50 (MQ=255)                                          gggTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACat  >  1:2285235/1‑50 (MQ=255)                                          gggTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACat  >  1:1785955/1‑50 (MQ=255)                                             |                                               GTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACAT  >  NC_000913/12042‑12132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑