Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,829,233	C→A	L97M (CTG→ATG)	xanP →	xanthine:H(+) symporter XanP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,829,233	0	C	A	100.0%	49.5 / NA	15	L97M (CTG→ATG)	xanP	xanthine:H(+) symporter XanP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (3/12);  total (3/12)

TGGCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGCTGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTG  >  NC_000913/3829186‑3829277                                                |                                             tgGCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGAtg                                            >  1:2061990/1‑50 (MQ=255)     aTCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGAtgttgt                                        <  1:860200/50‑1 (MQ=255)        gATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCt                                      <  1:1677837/49‑1 (MQ=255)         attattCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTAt                                    <  1:406272/50‑1 (MQ=255)          ttattCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTAtt                                   <  1:110230/50‑1 (MQ=255)                aaaTTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAggg                              <  1:935946/49‑1 (MQ=255)                   ttAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCAc                           >  1:453856/1‑49 (MQ=255)                     aaGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCa                         <  1:1821458/49‑1 (MQ=255)                          cTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCa                   <  1:1435558/50‑1 (MQ=255)                           tGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCaa                  >  1:216784/1‑50 (MQ=255)                                tCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTttg             <  1:38468/50‑1 (MQ=255)                                  cGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTttgt            <  1:114030/49‑1 (MQ=255)                                       ggCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGccc       <  1:948953/49‑1 (MQ=255)                                            cGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCt   <  1:1949255/48‑1 (MQ=255)                                            cGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTg  <  1:2283327/49‑1 (MQ=255)                                                |                                             TGGCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGCTGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTG  >  NC_000913/3829186‑3829277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑