Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,696	C→T	G103D (GGC→GAC)	asnC ←	DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,696	0	C	T	100.0%	107.6 / NA	30	G103D (GGC→GAC)	asnC	DNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/17);  total (13/17)

GCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTT  >  NC_000913/3926651‑3926742                                              |                                               gCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGt                                            <  1:2320247/50‑1 (MQ=255)      gatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTgtgt                                       <  1:1101936/50‑1 (MQ=255)      gatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTgtgt                                       <  1:1752867/50‑1 (MQ=255)        tcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTaa                                     <  1:1445848/50‑1 (MQ=255)          gaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTaa                                     >  1:2070744/1‑48 (MQ=255)           aaCGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAg                                  >  1:1203490/1‑50 (MQ=255)            aCGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAgg                                 >  1:1313777/1‑50 (MQ=255)             cGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGc                                >  1:177206/1‑50 (MQ=255)              ggCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCt                               >  1:929429/1‑50 (MQ=255)                cacaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTc                             <  1:1633166/50‑1 (MQ=255)                cacaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTc                             <  1:472736/50‑1 (MQ=255)                  caTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAg                           <  1:1957330/50‑1 (MQ=255)                   aTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGt                          >  1:1468877/1‑50 (MQ=255)                    tCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTg                         <  1:577335/50‑1 (MQ=255)                     cACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGa                        >  1:40220/1‑50 (MQ=255)                       cTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACt                      >  1:1010192/1‑50 (MQ=255)                          ttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACtt                     <  1:1492727/48‑1 (MQ=255)                          ttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtc                    <  1:761560/49‑1 (MQ=255)                             taAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc                 >  1:227918/1‑49 (MQ=255)                             taAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc                 <  1:2280965/49‑1 (MQ=255)                               aaGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAg              >  1:530871/1‑50 (MQ=255)                                  aTGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCt           <  1:579377/50‑1 (MQ=255)                                    gCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCttt         >  1:2018813/1‑50 (MQ=255)                                     cTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTc        >  1:1144473/1‑50 (MQ=255)                                        tAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTcc       <  1:239625/48‑1 (MQ=255)                                        tAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTcc       <  1:2144831/48‑1 (MQ=255)                                        tAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAg     <  1:693838/50‑1 (MQ=255)                                          gTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCt   <  1:2178375/50‑1 (MQ=255)                                           tGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCtt  <  1:254873/50‑1 (MQ=255)                                           tGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCtt  >  1:1426412/1‑50 (MQ=255)                                              |                                               GCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTT  >  NC_000913/3926651‑3926742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑