Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,979,769	C→T	A390V (GCG→GTG)	wzyE →	enterobacterial common antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,979,769	0	C	T	100.0%	77.5 / NA	22	A390V (GCG→GTG)	wzyE	enterobacterial common antigen polymerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

GCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTT  >  NC_000913/3979722‑3979815                                                |                                               gCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGa                                              >  1:1851634/1‑50 (MQ=255)  ccTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGAt                                             <  1:1542076/50‑1 (MQ=255)       tCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCa                                        <  1:73271/50‑1 (MQ=255)       tCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCa                                        <  1:520134/50‑1 (MQ=255)         gCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAat                                      <  1:1903884/50‑1 (MQ=255)          cTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAata                                     <  1:1178736/50‑1 (MQ=255)            ataAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAatat                                    >  1:800703/1‑49 (MQ=255)             taAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATg                                   <  1:2237918/49‑1 (MQ=255)              aaaGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGAt                                 >  1:1427975/1‑50 (MQ=255)               aaGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATc                                <  1:1890516/50‑1 (MQ=255)                 gCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGt                              <  1:1051118/50‑1 (MQ=255)                 gCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGt                              <  1:588302/50‑1 (MQ=255)                 gCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGt                              <  1:86513/50‑1 (MQ=255)                  cTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTg                             <  1:2195816/50‑1 (MQ=255)                    gCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCt                           >  1:804311/1‑50 (MQ=255)                              acaGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTg                   >  1:2025128/1‑48 (MQ=255)                                  tttCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGc             >  1:1826775/1‑50 (MQ=255)                                   ttCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCt            >  1:1859810/1‑50 (MQ=255)                                    tCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTg           >  1:1973205/1‑50 (MQ=255)                                      tGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGa         >  1:908390/1‑50 (MQ=255)                                         tttGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTc      >  1:1877023/1‑50 (MQ=255)                                             gggTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGttt  <  1:1207823/50‑1 (MQ=255)                                                |                                               GCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTT  >  NC_000913/3979722‑3979815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑