Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,093,223	A→G	V72V (GTT→GTC)	rhaM ←	L‑rhamnose mutarotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,093,223	0	A	G	100.0%	92.5 / NA	25	V72V (GTT→GTC)	rhaM	L‑rhamnose mutarotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/10);  total (15/10)

GTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATCT  >  NC_000913/4093178‑4093269                                              |                                               gTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt                                            >  1:95163/1‑50 (MQ=255)      cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                       <  1:376271/50‑1 (MQ=255)       gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGc                                      >  1:399195/1‑50 (MQ=255)        ggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCt                                     <  1:2303105/50‑1 (MQ=255)         gCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                    <  1:1089349/50‑1 (MQ=255)          cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                   >  1:910512/1‑50 (MQ=255)           aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                  <  1:1562350/50‑1 (MQ=255)            tAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                  >  1:1334566/1‑49 (MQ=255)              aCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAc                               <  1:986602/50‑1 (MQ=255)                aTCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             >  1:1680987/1‑50 (MQ=255)                   ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt                          <  1:157381/50‑1 (MQ=255)                    gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCTACCGCAtt                         <  1:291465/50‑1 (MQ=255)                    gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAtt                         >  1:1304164/1‑50 (MQ=255)                     tCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  1:77540/1‑50 (MQ=255)                      cATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  1:850297/1‑49 (MQ=255)                         atatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                    >  1:1858201/1‑50 (MQ=255)                           atTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGt                  >  1:1361109/1‑50 (MQ=255)                           atTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGt                  >  1:1353795/1‑50 (MQ=255)                                  ccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAg           <  1:456505/50‑1 (MQ=255)                                      cGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt        >  1:1576390/1‑49 (MQ=255)                                      cGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt        >  1:1233704/1‑49 (MQ=255)                                        ttGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCaa     >  1:1202197/1‑50 (MQ=255)                                         tGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt    >  1:882929/1‑50 (MQ=255)                                           gCAGACATCAGTGCTGGCCACCGCATTCCAGCGTTCTTCAGATTCAAtct  <  1:425613/50‑1 (MQ=255)                                           gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtc   <  1:2345755/49‑1 (MQ=255)                                              |                                               GTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATCT  >  NC_000913/4093178‑4093269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑