Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,124	T→C	W140R (TGG→CGG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,124	0	T	C	100.0%	108.4 / NA	29	W140R (TGG→CGG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/18);  total (11/18)

TTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438075‑4438169                                                  |                                              tttCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCt                                               <  1:2176851/50‑1 (MQ=255)    cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  1:1974421/1‑49 (MQ=255)        aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        >  1:1197525/1‑50 (MQ=255)           cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                     <  1:45762/50‑1 (MQ=255)            gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:2207771/1‑50 (MQ=255)               aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                  <  1:141776/49‑1 (MQ=255)               aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 <  1:1100085/50‑1 (MQ=255)                  ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               >  1:2233004/1‑49 (MQ=255)                    ccGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                             >  1:573611/1‑49 (MQ=255)                      gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc                          <  1:1131573/50‑1 (MQ=255)                       ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:527497/1‑48 (MQ=255)                          gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                       <  1:898773/49‑1 (MQ=255)                          gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                       <  1:756234/49‑1 (MQ=255)                           aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:2137188/50‑1 (MQ=255)                           aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:785822/50‑1 (MQ=255)                           aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:2297568/50‑1 (MQ=255)                            aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  1:464906/1‑49 (MQ=255)                            aCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg                    <  1:1494694/50‑1 (MQ=255)                             cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   <  1:155316/50‑1 (MQ=255)                             cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:2099442/1‑50 (MQ=255)                              aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGAc                  <  1:876501/50‑1 (MQ=255)                                 aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               >  1:2214278/1‑50 (MQ=255)                                   aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             <  1:1748391/50‑1 (MQ=255)                                     aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            >  1:1442592/1‑49 (MQ=255)                                      cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGa           >  1:637104/1‑49 (MQ=255)                                       gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa         <  1:302900/50‑1 (MQ=255)                                         ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       <  1:400407/50‑1 (MQ=255)                                              tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt   <  1:134031/49‑1 (MQ=255)                                              tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt   <  1:636925/49‑1 (MQ=255)                                              tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  <  1:43889/50‑1 (MQ=255)                                                  |                                              TTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438075‑4438169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑