Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	612,647	C→T	intergenic (‑153/‑90)	fepA ← / → fes	ferric enterobactin outer membrane transporter/enterochelin esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	612,647	0	C	T	100.0%	112.6 / NA	32	intergenic (‑153/‑90)	fepA/fes	ferric enterobactin outer membrane transporter/enterochelin esterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/19);  total (13/19)

AGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATT  >  NC_000913/612600‑612692                                                |                                              agtgcagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTATGc                                             >  1:347225/1‑50 (MQ=255)     cagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTATGCTAtt                                         >  1:641083/1‑50 (MQ=255)     cagtTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTATGCTAt                                          <  1:1402003/49‑1 (MQ=255)        ttGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTATGCTATtgca                                      <  1:1592214/50‑1 (MQ=255)           gTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTATGCTATtgcaaat                                   <  1:545527/50‑1 (MQ=255)           gTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTATGCTATtgcaaat                                   <  1:402549/50‑1 (MQ=255)            ttaattaaTGTCCGCGCTTCCCACGGCGCGCCATTATGCTATtgcaaatg                                  >  1:1708123/1‑50 (MQ=255)             taattaatGTCCGCGCTTCCCACGGCGCGCCATTATGCTATtgcaaatg                                  >  1:2286940/1‑49 (MQ=255)              aattaatGTCCGCGCTTCCCACGGCGCGCCATTATGCTATtgcaaatgc                                 <  1:861183/49‑1 (MQ=255)               attaatGTCCGCGCTTCCCACGGCGCGCCATTATGCTATtgcaaatgca                                <  1:587534/49‑1 (MQ=255)               attaatGTCCGCGCTTCCCACGGCGCGCCATTATGCTATtgcaaatgca                                <  1:1680085/49‑1 (MQ=255)                ttaatGTCCGCGCTTCCCACGGCGCGCCATTATGCTATtgcaaatgcaaa                              <  1:96356/50‑1 (MQ=255)                   atGTCCGCGCTTCCCACGGCGCGCCATTATGCTATTGCAAATGCAAATa                            <  1:1130724/49‑1 (MQ=255)                    tGTCCGCGCTTCCCACGGCGCGCCATTATGCTATTGCAAATGCAAATAGt                          <  1:869974/50‑1 (MQ=255)                    tGTCCGCGCTTCCCACGGCGCGCCATTATGCTATTGCAAATGCAAATAGt                          <  1:439379/50‑1 (MQ=255)                      tCCGCGCTTCCCACGGCGCGCCATTATGCTATTGCAAATGCAAATAGTTa                        >  1:786236/1‑50 (MQ=255)                       ccGCGCTTCCCACGGCGCGCCATTATGCTATTGCAAATGCAAATAGTTAt                       >  1:601362/1‑50 (MQ=255)                        cgcgCTTCCCACGGCGCGCCATTATGCTATTGCAAATGCAAATAGTTAt                       <  1:2332642/49‑1 (MQ=255)                               cccACGGCGCGCCATTATGCTATTGCAAATGCAAATAGTTATCaataa                 <  1:1068731/48‑1 (MQ=255)                               cccACGGCGCGCCATTATGCTATTGCAAATGCAAATAGTTATCAataata               >  1:1780256/1‑50 (MQ=255)                                ccACGGCGCGCCATTATGCTATTGCAAATGCAAATAGTTATCAATAatat              <  1:95904/50‑1 (MQ=255)                                  aCGGCGCGCCATTATGCTATTGCAAATGCAAATAGTTATCAATAAtatta            <  1:1158192/50‑1 (MQ=255)                                  aCGGCGCGCCATTATGCTATTGCAAATGCAAATAGTTATCAATAAtatta            >  1:735183/1‑50 (MQ=255)                                    ggCGCGCCATTATGCTATTGCAAATGCAAATAGTTATCAATAATATTATc          <  1:37365/50‑1 (MQ=255)                                     gcgcgcCATTATGCTATTGCAAATGCAAATAGTTATCAATAATATTATc          >  1:167104/1‑49 (MQ=255)                                     gcgcgcCATTATGCTATTGCAAATGCAAATAGTTATCAATAATATTATCa         <  1:878744/50‑1 (MQ=255)                                      cgcgcCATTATGCTATTGCAAATGCAAATACTTATCAATAATATTATCaa        >  1:1137436/1‑50 (MQ=255)                                        cgcCATTATGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAata      >  1:2056115/1‑50 (MQ=255)                                         gcCATTATGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAata      >  1:408807/1‑49 (MQ=255)                                         gcCATTATGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAata      >  1:2123346/1‑49 (MQ=255)                                            aTTATGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATAtt  <  1:1229024/50‑1 (MQ=255)                                            aTTATGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATAtt  <  1:1390589/50‑1 (MQ=255)                                                |                                              AGTGCAGTTGGTTAATTAATGTCCGCGCTTCCCACGGCGCGCCATTACGCTATTGCAAATGCAAATAGTTATCAATAATATTATCAATATATT  >  NC_000913/612600‑612692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑