| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 978,231 | C→T | L636L (CTG→TTG) | mukB → | chromosome partitioning protein MukB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 978,231 | 0 | C | T | 89.5% | 49.3 / 0.0 | 19 | L636L (CTG→TTG) | mukB | chromosome partitioning protein MukB |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (8/9); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
AGAGTTTACCTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAG > NC_000913/978185‑978270 | agagTTTACCTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGTTgg < 1:558505/50‑1 (MQ=255) agTTTACCTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGTTGGAg > 1:1648124/1‑50 (MQ=255) ccTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGa < 1:971104/49‑1 (MQ=255) cTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGTTGGAGCGTGAGc < 1:127786/50‑1 (MQ=255) cTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGTTGGAGCGTGAGc > 1:1494152/1‑50 (MQ=255) gccagGACGTCACCGAATATCTGCAACAGTTGTTGGAGCGTGAGCGagag < 1:1693696/50‑1 (MQ=255) aCGTCACCGAATATCTGCAACAGTTGTTGGAGCGTGAGCGAGAGGCGAtt > 1:75885/1‑50 (MQ=255) aCGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGAtt < 1:920696/50‑1 (MQ=255) cGTCACCGAATATCTGCAACAGTTGTTGGAGCGTGAGCGAGAGGCGAttg < 1:962537/50‑1 (MQ=255) gTCACCGAATATCTGCAACAGTTGTTGGAGCGTGAGCGAGAGGCGAttgt > 1:2296596/1‑50 (MQ=255) aCCGAATATCTGCAACAGTTGTTGGAGCGTGAGCGAGAGGCGATTGTTGa < 1:2197848/50‑1 (MQ=255) ccGAATATCTGCAACAGTTGTTGGAGCGTGAGCGAGAGGCGATTGTTGaa < 1:2164998/50‑1 (MQ=255) cGAATATCTGCAACAGTTGTTGGAGCGTGAGCGAGAGGCGATTGTTGAAc > 1:600998/1‑50 (MQ=255) cGAATATCTGCAACAGTTGTTGGAGCGTGAGCGAGAGGCGATTGTTGAAc < 1:660724/50‑1 (MQ=255) gAATATCTGCAACAGTTGTTGGAGCGTGAGCGAGAGGCGATTGTTGAAcg < 1:2193756/50‑1 (MQ=255) atCTGCAACAGTTGTTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGAt > 1:1946943/1‑50 (MQ=255) atCTGCAACAGTTGTTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGAt > 1:955508/1‑50 (MQ=255) cTGCAACAGTTGTTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATg > 1:2061317/1‑49 (MQ=255) cAACAGTTGTTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAg < 1:1122172/49‑1 (MQ=255) | AGAGTTTACCTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAG > NC_000913/978185‑978270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |