Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	97.6 / NA	28	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/16);  total (12/16)

GAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988455‑988545                                             |                                               gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:986746/1‑49 (MQ=255)    cATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaaa                                        <  1:816498/50‑1 (MQ=255)    cATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaaa                                        <  1:1722750/50‑1 (MQ=255)        tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                     <  1:1605028/49‑1 (MQ=255)          gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:799054/1‑49 (MQ=255)          gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:1442889/1‑49 (MQ=255)          gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   <  1:2273002/49‑1 (MQ=255)            cTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:456184/1‑47 (MQ=255)              gCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAgaagaa                              >  1:2071355/1‑50 (MQ=255)               cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaaga                               <  1:598045/48‑1 (MQ=255)                aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            <  1:620219/50‑1 (MQ=255)                      ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                       <  1:1022106/49‑1 (MQ=255)                      ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                       <  1:252715/49‑1 (MQ=255)                           cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  >  1:1662754/1‑49 (MQ=255)                              aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              <  1:1702143/50‑1 (MQ=255)                               tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaaga             <  1:1558550/50‑1 (MQ=255)                                 cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag           >  1:985594/1‑50 (MQ=255)                                 cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag           >  1:1197837/1‑50 (MQ=255)                                 cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaa            <  1:314116/49‑1 (MQ=255)                                  ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          <  1:808249/50‑1 (MQ=255)                                  ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  1:1134607/1‑50 (MQ=255)                                  ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          <  1:772522/50‑1 (MQ=255)                                  ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  1:738890/1‑50 (MQ=255)                                  ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          <  1:668425/50‑1 (MQ=255)                                       tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  1:481611/1‑49 (MQ=255)                                         aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCa    <  1:1135507/49‑1 (MQ=255)                                         aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   <  1:337050/50‑1 (MQ=255)                                          gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  >  1:386835/1‑50 (MQ=255)                                             |                                               GAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988455‑988545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑