Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,362	C→T	A84V (GCC→GTC)	mltG →	endolytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,362	0	C	T	100.0%	84.1 / NA	24	A84V (GCC→GTC)	mltG	endolytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/9);  total (15/9)

CAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTG  >  NC_000913/1154319‑1154408                                            |                                               cAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGa                                           <  1:2190260/49‑1 (MQ=255)      gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                     <  1:745996/50‑1 (MQ=255)      gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                     <  1:255908/50‑1 (MQ=255)           tgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCtt                                 >  1:811503/1‑49 (MQ=255)           tgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCtt                                 >  1:88479/1‑49 (MQ=255)             cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTa                               <  1:1013983/49‑1 (MQ=255)             cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTa                               >  1:1886441/1‑49 (MQ=255)              gTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTaca                             >  1:682170/1‑50 (MQ=255)              gTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTaca                             >  1:2337828/1‑50 (MQ=255)                aTCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACAcc                           >  1:900194/1‑50 (MQ=255)                    aaCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:1190874/1‑47 (MQ=255)                    aaCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:1611056/1‑47 (MQ=255)                         gATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAc                  <  1:690577/50‑1 (MQ=255)                           tCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCg                >  1:1364218/1‑50 (MQ=255)                            cTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt               >  1:1236306/1‑50 (MQ=255)                              ttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgc             >  1:2130574/1‑50 (MQ=255)                               tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTg              <  1:497417/48‑1 (MQ=255)                                ctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcg            <  1:1462249/49‑1 (MQ=255)                                 tcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc           >  1:252004/1‑49 (MQ=255)                                 tcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc           >  1:1725719/1‑49 (MQ=255)                                   aCTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc           >  1:863466/1‑47 (MQ=255)                                   aCTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCga         <  1:1335702/49‑1 (MQ=255)                                         aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCt   >  1:912534/1‑49 (MQ=255)                                         aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTg  <  1:53308/50‑1 (MQ=255)                                            |                                               CAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTG  >  NC_000913/1154319‑1154408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑