Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	83.3 / NA	23	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/15);  total (8/15)

GCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382510‑1382601                                              |                                               gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGc                                             >  1:1069594/1‑49 (MQ=255)   gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           >  1:1716817/1‑49 (MQ=255)     gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        <  1:2030497/50‑1 (MQ=255)      tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:406604/50‑1 (MQ=255)      tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:1857935/50‑1 (MQ=255)          cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:1095035/1‑49 (MQ=255)             ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                 <  1:887200/49‑1 (MQ=255)             ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                                >  1:638907/1‑50 (MQ=255)                   cGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                          >  1:2126623/1‑50 (MQ=255)                    gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         <  1:1429721/50‑1 (MQ=255)                     aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        <  1:1880519/50‑1 (MQ=255)                         cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    <  1:1646114/50‑1 (MQ=255)                          ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    >  1:449688/1‑49 (MQ=255)                          ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:150285/50‑1 (MQ=255)                              tCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt               >  1:1557604/1‑50 (MQ=255)                                aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg             >  1:657146/1‑50 (MQ=255)                                aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:2227854/49‑1 (MQ=255)                                aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACACAAGAAATTc              <  1:2084273/49‑1 (MQ=255)                                  tatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgcg           <  1:553127/50‑1 (MQ=255)                                    tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         <  1:161282/50‑1 (MQ=255)                                        cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     <  1:1935151/50‑1 (MQ=255)                                         gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    <  1:1092707/50‑1 (MQ=255)                                           cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:2181725/50‑1 (MQ=255)                                              |                                               GCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382510‑1382601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑