Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,028	T→C	V99A (GTC→GCC)	ampD →	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,028	0	T	C	100.0%	79.2 / NA	22	V99A (GTC→GCC)	ampD	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/8);  total (14/8)

TAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTG  >  NC_000913/118983‑119072                                              |                                             tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                          <  1:354219/50‑1 (MQ=255)   gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                          >  1:100393/1‑48 (MQ=255)   gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                          >  1:568622/1‑48 (MQ=255)    tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                       >  1:1175908/1‑50 (MQ=255)      cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                     >  1:1468463/1‑50 (MQ=255)      cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                     <  1:507666/50‑1 (MQ=255)       aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                    <  1:1393420/50‑1 (MQ=255)       aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                    >  1:1507894/1‑50 (MQ=255)               ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg                            >  1:1029517/1‑50 (MQ=255)               ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                             <  1:1038917/49‑1 (MQ=255)                cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                           >  1:1652948/1‑50 (MQ=255)                      aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                     >  1:271945/1‑50 (MQ=255)                      aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                     >  1:263293/1‑50 (MQ=255)                        aaaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                   >  1:1555201/1‑50 (MQ=255)                         aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                   >  1:418638/1‑49 (MQ=255)                         aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                  <  1:67124/50‑1 (MQ=255)                           cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                <  1:1693210/50‑1 (MQ=255)                              gcatggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa             >  1:1158495/1‑50 (MQ=255)                               catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt            >  1:1835184/1‑50 (MQ=255)                               catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt            <  1:1306042/50‑1 (MQ=255)                                     atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc       >  1:1976837/1‑49 (MQ=255)                                     atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt      <  1:1721156/50‑1 (MQ=255)                                     atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt      <  1:1704268/50‑1 (MQ=255)                                        cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAtt   >  1:480215/1‑50 (MQ=255)                                         gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg  >  1:617833/1‑50 (MQ=255)                                              |                                             TAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTG  >  NC_000913/118983‑119072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑