| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,562,085 | C→T | A133T (GCA→ACA) | ddpA ← | putative D,D‑dipeptide ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,562,085 | 0 | C | T | 96.8% | 105.2 / ‑5.7 | 31 | A133T (GCA→ACA) | ddpA | putative D,D‑dipeptide ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (18/12); total (19/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAG > NC_000913/1562039‑1562130 | aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTc < 1:1760998/50‑1 (MQ=255) ccGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTct < 1:1491556/50‑1 (MQ=255) cGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctg < 1:1089679/50‑1 (MQ=255) gTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgc > 1:1068785/1‑50 (MQ=255) aTGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgctg > 1:2057789/1‑50 (MQ=255) gTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGc > 1:2044425/1‑50 (MQ=255) ttCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcc < 1:800950/50‑1 (MQ=255) ttCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcc < 1:2162359/50‑1 (MQ=255) cgtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcccc > 1:1088606/1‑50 (MQ=255) cgtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcccc > 1:818086/1‑50 (MQ=255) gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcccc < 1:444039/49‑1 (MQ=255) gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt < 1:1789658/50‑1 (MQ=255) cgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTgg > 1:209991/1‑50 (MQ=255) gggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGc > 1:2342906/1‑50 (MQ=255) ggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGcc > 1:1078772/1‑50 (MQ=255) gAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGcc > 1:2128003/1‑49 (MQ=255) cATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtt < 1:1116154/50‑1 (MQ=255) cAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt > 1:2324148/1‑49 (MQ=255) tCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag > 1:835188/1‑49 (MQ=255) tCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag > 1:1834256/1‑49 (MQ=255) tttAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag > 1:346096/1‑50 (MQ=255) tttAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag > 1:657789/1‑50 (MQ=255) ttAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGc > 1:1451547/1‑50 (MQ=255) tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagt < 1:2132679/46‑1 (MQ=255) aaaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCg > 1:1057997/1‑50 (MQ=255) aTCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCt > 1:338796/1‑50 (MQ=255) cTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTc > 1:2303371/1‑49 (MQ=255) tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaaa < 1:328301/49‑1 (MQ=255) tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa < 1:2205465/48‑1 (MQ=255) ggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga < 1:2103561/50‑1 (MQ=255) aaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaagaaag > 1:1092007/1‑50 (MQ=255) | ACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAG > NC_000913/1562039‑1562130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |