Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,662,872	C→T	R773W (CGG→TGG)	ynfF →	putative selenate reductase YnfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,662,872	0	C	T	100.0%	65.6 / NA	19	R773W (CGG→TGG)	ynfF	putative selenate reductase YnfF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/9);  total (10/9)

CCGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCGCCC  >  NC_000913/1662825‑1662918                                                |                                               ccGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTgg                                              <  1:1002510/50‑1 (MQ=255)       tCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                        <  1:1849549/50‑1 (MQ=255)        cGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                        >  1:1605303/1‑49 (MQ=255)           gCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcg                                    <  1:126270/50‑1 (MQ=255)                gTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggcgg                                >  1:431563/1‑49 (MQ=255)                   cGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAg                             >  1:1114729/1‑49 (MQ=255)                      ggCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTAt                          >  1:1065380/1‑49 (MQ=255)                      ggCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTAt                          >  1:1527404/1‑49 (MQ=255)                       gCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATc                         >  1:1102473/1‑49 (MQ=255)                       gCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATc                         <  1:575414/49‑1 (MQ=255)                       gCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATc                         <  1:1166124/49‑1 (MQ=255)                               cGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCt                 >  1:197211/1‑49 (MQ=255)                               cGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTg                <  1:1044343/50‑1 (MQ=255)                               cGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTg                <  1:1360529/50‑1 (MQ=255)                                ggATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGa               >  1:946436/1‑50 (MQ=255)                                   tatGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACct            >  1:1141488/1‑50 (MQ=255)                                     tGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACctct          <  1:1918962/50‑1 (MQ=255)                                       tttGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCa        <  1:958564/50‑1 (MQ=255)                                             gACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCGccc  >  1:1645703/1‑50 (MQ=255)                                                |                                               CCGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCGCCC  >  NC_000913/1662825‑1662918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑