Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,697,216	T→C	intergenic (‑164/+57)	uidR ← / ← hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,697,216	0	T	C	100.0%	114.9 / NA	30	intergenic (‑164/+57)	uidR/hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/17);  total (13/17)

AACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTC  >  NC_000913/1697173‑1697262                                            |                                               aaCCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgat                                            >  1:285287/1‑48 (MQ=255)    cAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGtt                                       >  1:845781/1‑50 (MQ=255)     aaTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGttt                                      <  1:810655/50‑1 (MQ=255)      aTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTa                                     >  1:1134919/1‑50 (MQ=255)      aTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTa                                     >  1:1585018/1‑50 (MQ=255)       tAGCCGTGAGTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTAt                                    <  1:2003790/50‑1 (MQ=39)           cGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCg                                <  1:221505/50‑1 (MQ=255)           cGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCg                                <  1:1598448/50‑1 (MQ=255)            gTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCgg                               <  1:848912/50‑1 (MQ=255)             tGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGt                              <  1:562593/50‑1 (MQ=255)               aTTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaa                            <  1:1114554/50‑1 (MQ=255)               aTTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTa                             >  1:1684433/1‑49 (MQ=255)                 ttGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaaa                           >  1:1817506/1‑49 (MQ=255)                    gCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaacaa                        <  1:2155186/49‑1 (MQ=255)                     cTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAacaaca                      >  1:1362625/1‑50 (MQ=255)                       gCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacg                    >  1:624665/1‑50 (MQ=255)                        ccAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgc                   <  1:1697752/50‑1 (MQ=255)                          aGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac                 >  1:1842984/1‑50 (MQ=255)                           gTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcacg                <  1:2188330/50‑1 (MQ=255)                            ttATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACgg               <  1:1038345/50‑1 (MQ=255)                            ttATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACgg               <  1:2093162/50‑1 (MQ=255)                              aTTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgtt             >  1:1252464/1‑50 (MQ=255)                               tttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttg            <  1:989683/50‑1 (MQ=255)                               tttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttg            >  1:2280776/1‑50 (MQ=255)                               tttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgtt             <  1:1435708/49‑1 (MQ=255)                                 tAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgtt          >  1:202198/1‑50 (MQ=255)                                    cAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAc       <  1:553749/50‑1 (MQ=255)                                      gagaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCg     <  1:141186/50‑1 (MQ=255)                                        gaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGt   >  1:139974/1‑50 (MQ=255)                                         aCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTc  <  1:1655263/50‑1 (MQ=255)                                            |                                               AACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTC  >  NC_000913/1697173‑1697262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑