Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,741,062	T→C	C384C (TGT→TGC)	ydhC →	putative transporter YdhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,741,062	0	T	C	100.0%	67.9 / NA	19	C384C (TGT→TGC)	ydhC	putative transporter YdhC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/7);  total (12/7)

TTATCAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCA  >  NC_000913/1741015‑1741106                                                |                                             ttATCAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCga                                            <  1:2067652/50‑1 (MQ=255)        cAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGtt                                     >  1:541135/1‑50 (MQ=255)             gtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCt                                 <  1:2271621/49‑1 (MQ=255)             gtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCt                                 <  1:1712638/49‑1 (MQ=255)             gtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTg                                <  1:1122444/50‑1 (MQ=255)                  ggTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAg                            >  1:829773/1‑49 (MQ=255)                   gTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGa                           <  1:1892741/49‑1 (MQ=255)                    tCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAAt                         <  1:2190010/50‑1 (MQ=255)                      gcgcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATc                        <  1:301585/49‑1 (MQ=255)                       cgcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCAt                      >  1:1509595/1‑50 (MQ=255)                        gcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATg                     >  1:2088577/1‑50 (MQ=255)                          tGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATgg                    >  1:1371026/1‑49 (MQ=255)                               tACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGc              >  1:1889855/1‑50 (MQ=255)                                  atgatgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCg            >  1:624/1‑49 (MQ=255)                                    gatgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAg         >  1:1960936/1‑50 (MQ=255)                                    gatgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAg         >  1:1260994/1‑50 (MQ=255)                                      tgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTc       >  1:55789/1‑50 (MQ=255)                                          aCGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCt    >  1:1793963/1‑49 (MQ=255)                                            gTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCa  >  1:700369/1‑49 (MQ=255)                                                |                                             TTATCAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCA  >  NC_000913/1741015‑1741106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑