Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	100.1 / NA	27	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (16/11);  total (16/11)

GCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742712‑1742800                                            |                                              gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          >  1:1746776/1‑49 (MQ=255)  ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                                        <  1:2082667/50‑1 (MQ=255)   cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                       <  1:2006346/50‑1 (MQ=255)     aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                     >  1:467592/1‑50 (MQ=255)      gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                     >  1:34246/1‑49 (MQ=255)       ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                   >  1:78834/1‑50 (MQ=255)       ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                   >  1:470277/1‑50 (MQ=255)        tttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  >  1:633195/1‑50 (MQ=255)                     gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     <  1:1991224/50‑1 (MQ=255)                        tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  >  1:1949741/1‑50 (MQ=255)                        tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  <  1:148335/50‑1 (MQ=255)                        tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  <  1:674526/50‑1 (MQ=255)                          gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:74865/1‑50 (MQ=255)                           tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg               <  1:360327/50‑1 (MQ=255)                            gCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa              >  1:1534446/1‑50 (MQ=255)                             cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc             <  1:1739452/50‑1 (MQ=255)                             cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc             <  1:2023054/50‑1 (MQ=255)                              gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:2129493/1‑50 (MQ=255)                               ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           >  1:481567/1‑50 (MQ=255)                               ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           >  1:1104273/1‑50 (MQ=255)                               ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           >  1:1418331/1‑50 (MQ=255)                                tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          >  1:1793500/1‑50 (MQ=255)                                   aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       <  1:94817/50‑1 (MQ=255)                                    gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCg      <  1:130433/50‑1 (MQ=255)                                     tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa     >  1:1087569/1‑50 (MQ=255)                                       tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg   <  1:625238/50‑1 (MQ=255)                                        ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg  >  1:1135123/1‑50 (MQ=255)                                            |                                              GCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742712‑1742800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑