Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,533	G→A	G316D (GGC→GAC)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,533	0	G	A	100.0%	111.7 / NA	32	G316D (GGC→GAC)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/21);  total (11/21)

AAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACG  >  NC_000913/1776488‑1776579                                              |                                               aaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTg                                            <  1:1399085/50‑1 (MQ=255)  aGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTgg                                           <  1:2064497/50‑1 (MQ=255)    gcgcGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTc                                         >  1:1270065/1‑50 (MQ=255)       cgGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGcc                                      >  1:1347931/1‑50 (MQ=255)        ggTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                     <  1:240786/50‑1 (MQ=255)        ggTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                     >  1:1771646/1‑50 (MQ=255)         gTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTGGCCCg                                    <  1:166897/50‑1 (MQ=255)         gTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCg                                    <  1:2005121/50‑1 (MQ=255)              gAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCgag                                  >  1:1995120/1‑47 (MQ=255)              gAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGagaag                               <  1:2102963/50‑1 (MQ=255)               aaTGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGagaaga                              <  1:96521/50‑1 (MQ=255)                     ggCGTCGGTATTGCTGACGGCATTGATCTGGTCGCCCGAGAAGAAGGTtg                        >  1:1008784/1‑50 (MQ=38)                     ggCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGtt                         <  1:1679076/49‑1 (MQ=255)                     ggCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtg                        >  1:2167309/1‑50 (MQ=255)                      gCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgt                       <  1:1456857/50‑1 (MQ=255)                       cGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgtg                      <  1:368262/50‑1 (MQ=255)                        gTCGGTATTGCTGACGGGATTGACCTGGTCGCCCGAGAAGAAGGTTGTGc                     <  1:1296285/50‑1 (MQ=38)                           ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtga                  >  1:156640/1‑50 (MQ=255)                           ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtg                   <  1:1241580/49‑1 (MQ=255)                            gTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgat                 <  1:1522575/50‑1 (MQ=255)                               ttGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGAc              <  1:1456860/50‑1 (MQ=255)                                   tGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTa          >  1:170594/1‑50 (MQ=255)                                     aCGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTa          <  1:2269912/48‑1 (MQ=255)                                     aCGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTa          <  1:261886/48‑1 (MQ=255)                                      cGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTc       >  1:1068112/1‑50 (MQ=255)                                       ggCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCt      <  1:1848300/50‑1 (MQ=255)                                       ggCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCt      >  1:470074/1‑50 (MQ=255)                                       ggCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCt      <  1:518291/50‑1 (MQ=255)                                          aTTGACCTGGTCGCCCGAGGAGAAGGTTGTGCTGATGACTTTATTCTGAc   <  1:1274368/50‑1 (MQ=255)                                          aTTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAc   <  1:1796572/50‑1 (MQ=255)                                          aTTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAc   <  1:101876/50‑1 (MQ=255)                                           ttGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACg  >  1:565384/1‑50 (MQ=255)                                              |                                               AAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACG  >  NC_000913/1776488‑1776579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑