Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,208	T→C	V92A (GTT→GCT)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,208	0	T	C	100.0%	64.3 / NA	18	V92A (GTT→GCT)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/14);  total (4/14)

GTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTT  >  NC_000913/1811171‑1811250                                      |                                           gTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGc                                 <  1:1402230/49‑1 (MQ=255)     tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATaa                             <  1:772973/49‑1 (MQ=255)     tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                            <  1:205136/50‑1 (MQ=255)       ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGc                          <  1:2187804/50‑1 (MQ=255)         gTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                        >  1:1639791/1‑50 (MQ=255)          ttAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtc                       >  1:296226/1‑50 (MQ=255)          ttAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtc                       <  1:1018680/50‑1 (MQ=255)            aGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                      <  1:1465834/49‑1 (MQ=255)             gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                      >  1:791454/1‑48 (MQ=255)             gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCa                    <  1:255191/50‑1 (MQ=255)              tCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAg                   <  1:1064020/50‑1 (MQ=255)                  cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTa                <  1:2169351/49‑1 (MQ=255)                  cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTa                <  1:1093307/49‑1 (MQ=255)                   gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAg               <  1:1393206/49‑1 (MQ=255)                    ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg              >  1:810464/1‑49 (MQ=255)                     ctctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCa            <  1:1268987/50‑1 (MQ=255)                          ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa         <  1:2262511/48‑1 (MQ=255)                               aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGttt  <  1:252621/50‑1 (MQ=255)                                      |                                           GTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTT  >  NC_000913/1811171‑1811250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑