Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,898,152	G→A	A407A (GCG→GCA)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,898,152	0	G	A	100.0%	53.0 / NA	16	A407A (GCG→GCA)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/10);  total (6/10)

TTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATA  >  NC_000913/1898109‑1898198                                            |                                               ttGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATg                                          <  1:1749667/50‑1 (MQ=255)   gAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATgg                                         <  1:862671/49‑1 (MQ=255)     gCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctc                                      <  1:1584467/50‑1 (MQ=255)           tGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGcgcc                                <  1:2349461/50‑1 (MQ=255)              cATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCa                             >  1:1652187/1‑50 (MQ=255)              cATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCa                             <  1:2221956/50‑1 (MQ=255)                    ctctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTg                       <  1:224487/50‑1 (MQ=255)                        gtgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCAcc                   >  1:1805268/1‑50 (MQ=255)                         tgAAGGCGATTAACGCCGCACGGATGGCTCTGGGCCGCACCAGTGCAcc                   <  1:171725/49‑1 (MQ=37)                           aaGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACcgcg                <  1:1728968/50‑1 (MQ=255)                               cGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtc            >  1:282015/1‑50 (MQ=255)                                gATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtct           >  1:2168722/1‑50 (MQ=255)                                gATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtct           <  1:1645186/50‑1 (MQ=255)                                gATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtct           >  1:1198150/1‑50 (MQ=255)                                 aTTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc          <  1:2275757/50‑1 (MQ=255)                                     aCGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTg      >  1:1044193/1‑50 (MQ=255)                                         cgcacgGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATa  <  1:2186057/50‑1 (MQ=255)                                            |                                               TTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATA  >  NC_000913/1898109‑1898198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑