Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,885	C→T	T18I (ACC→ATC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,885	0	C	T	100.0%	85.8 / NA	24	T18I (ACC→ATC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/15);  total (9/15)

TGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAG  >  NC_000913/214838‑214928                                                |                                            tgtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                           >  1:2331586/1‑50 (MQ=255)    gATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCa                                        >  1:1450874/1‑50 (MQ=255)      tttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAtt                                      >  1:717415/1‑50 (MQ=255)        tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                    >  1:820910/1‑50 (MQ=255)          ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGt                                   <  1:177896/49‑1 (MQ=255)          ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                  <  1:2062339/50‑1 (MQ=255)           cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                 <  1:2327217/50‑1 (MQ=255)               aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                             <  1:201338/50‑1 (MQ=255)               aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                             <  1:2105556/50‑1 (MQ=255)               aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                             <  1:2253811/50‑1 (MQ=255)                 gTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAggg                           >  1:556278/1‑50 (MQ=255)                     cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                       <  1:233361/50‑1 (MQ=255)                      tATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                      >  1:389539/1‑50 (MQ=255)                        ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                    <  1:2175899/50‑1 (MQ=255)                        ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                    <  1:406890/50‑1 (MQ=255)                             gATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                 >  1:145018/1‑48 (MQ=255)                                 gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagca            <  1:2045367/49‑1 (MQ=255)                                 gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCAt           <  1:1807682/50‑1 (MQ=255)                                  tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg          <  1:2247790/50‑1 (MQ=255)                                    aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt        <  1:1317149/50‑1 (MQ=255)                                        tGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat     >  1:327739/1‑49 (MQ=255)                                         gagaTCCTCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat     <  1:1257670/48‑1 (MQ=38)                                          agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat     >  1:1883506/1‑47 (MQ=255)                                          agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAg  <  1:280669/50‑1 (MQ=255)                                                |                                            TGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAG  >  NC_000913/214838‑214928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑