Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,004	T→C	E43G (GAA→GGA)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,004	0	T	C	100.0%	145.4 / NA	38	E43G (GAA→GGA)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/28);  total (10/28)

CGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAA  >  NC_000913/2661960‑2662050                                             |                                               cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGa                                           <  1:2284745/50‑1 (MQ=255)  gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                          <  1:1078939/50‑1 (MQ=255)    tGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                          >  1:1194240/1‑48 (MQ=255)      taaaCCAGTCCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                      <  1:1191876/49‑1 (MQ=255)      gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAg                                       >  1:1008999/1‑49 (MQ=255)      gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAg                                       >  1:593115/1‑49 (MQ=255)      gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                      <  1:966402/50‑1 (MQ=255)      gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                      <  1:1794587/50‑1 (MQ=255)       aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                      >  1:1094170/1‑49 (MQ=255)           cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAggg                                 <  1:184932/50‑1 (MQ=255)              aCCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGaaa                              <  1:1159893/50‑1 (MQ=255)               ccATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAt                             >  1:232710/1‑50 (MQ=255)                    tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc                         <  1:945242/49‑1 (MQ=255)                    tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc                         <  1:62038/49‑1 (MQ=255)                     ttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt                        <  1:1996797/49‑1 (MQ=255)                      tACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTg                       <  1:919366/49‑1 (MQ=255)                       aCGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGAc                     >  1:1943176/1‑50 (MQ=255)                        cGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACg                    <  1:1482105/50‑1 (MQ=255)                         gCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGt                   <  1:34163/50‑1 (MQ=255)                          cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt                  <  1:132323/50‑1 (MQ=255)                           aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc                 >  1:2206334/1‑50 (MQ=255)                           aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc                 <  1:328030/50‑1 (MQ=255)                            gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg                <  1:484085/50‑1 (MQ=255)                            gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg                >  1:2209709/1‑50 (MQ=255)                            gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg                <  1:316634/50‑1 (MQ=255)                             aCGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg               <  1:1560795/50‑1 (MQ=255)                             aCGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg               <  1:2208779/50‑1 (MQ=255)                             aCGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg               <  1:1974439/50‑1 (MQ=255)                              cGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa              <  1:628902/50‑1 (MQ=255)                                ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa            >  1:1663822/1‑50 (MQ=255)                                 gAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa            >  1:745876/1‑49 (MQ=255)                                   aaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat         <  1:1931643/50‑1 (MQ=255)                                    aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATc        <  1:2226220/50‑1 (MQ=255)                                    aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATc        <  1:1730158/50‑1 (MQ=255)                                     aCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa       <  1:66448/50‑1 (MQ=255)                                     aCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa       <  1:1354457/50‑1 (MQ=255)                                      cAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg      <  1:400067/50‑1 (MQ=255)                                       aGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGc     <  1:395536/50‑1 (MQ=255)                                         ttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCa   <  1:338311/50‑1 (MQ=255)                                         ttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCa   <  1:2014121/50‑1 (MQ=255)                                          tgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCaa  >  1:66526/1‑50 (MQ=255)                                             |                                               CGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAA  >  NC_000913/2661960‑2662050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑