Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	220,099	A→G	intergenic (‑104/+14)	rcsF ← / ← metQ	outer membrane lipoprotein RcsF/L‑methionine/D‑methionine ABC transporter membrane anchored binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	220,099	0	A	G	100.0%	64.7 / NA	19	intergenic (‑104/+14)	rcsF/metQ	outer membrane lipoprotein RcsF/L‑methionine/D‑methionine ABC transporter membrane anchored binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/13);  total (6/13)

TGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACA  >  NC_000913/220052‑220144                                                |                                              tGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGgaa                                             <  1:665134/50‑1 (MQ=255)    cAAGACGGGCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGGAACAg                                          <  1:1045814/50‑1 (MQ=255)     aaGGCGGGCAAGCGCCCGTCCTGAATTATATTACAAATTGTGGGagcagc                                         <  1:1579061/50‑1 (MQ=255)         cGGGCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGGAACAGCCTaa                                     <  1:946805/50‑1 (MQ=255)            gCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGGAACAGCCTaaaaa                                  <  1:1686766/50‑1 (MQ=255)               aGCGCCCGTCCTGAATGATATTACAAATTGTGGGAACAGCCTAAAAATTa                               >  1:452844/1‑50 (MQ=255)                gcgcCCGTCCTGAATGATATTACAAATTGTGGGAACAGCCTAAAAATTAc                              <  1:2064666/50‑1 (MQ=255)                 cgcCCGTCCTGAATGATATTACAAATTGTGGGAACAGCCTAAAAATTAcc                             <  1:952119/50‑1 (MQ=255)                 cgcCCGTCCTGAATGATATTACAAATTGTGGGAACAGCCTAAAAATTAcc                             <  1:1687265/50‑1 (MQ=255)                  gcCCGTCCTGAATGATATTACAAATTGTGGGAACAGCCTAAAAATTACCa                            <  1:2173726/50‑1 (MQ=255)                        ccTGAATGATATTACAAATTGTGGGAACAGCCTAAAAATTACCAGCCtt                       >  1:448218/1‑49 (MQ=255)                         cTGAATGATATTACAAATTGTGGGAACAGCCTAAAAATTACCAGCCttt                      >  1:1125368/1‑49 (MQ=255)                            aaTGATATTACAAATTGTGGGAACAGCCTAAAAATTACCAGCCTTTAACa                  <  1:2149874/50‑1 (MQ=255)                                   ttACAAATTGTGGGAACAGCCTAAAAATTACCAGCCTTTAACAGCTccgc           >  1:1708796/1‑50 (MQ=255)                                      cAAATTGTGGGAACAGCCTAAAAATTACCAGCCTTTAACAGCTccgccg         <  1:1256634/49‑1 (MQ=255)                                      cAAATTGTGGGAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGt        >  1:1761329/1‑50 (MQ=255)                                      cAAATTGTGGGAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGt        <  1:193382/50‑1 (MQ=255)                                       aaaTTGTGGGAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGtt       <  1:1001251/50‑1 (MQ=255)                                       aaaTTGTGGGAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGt        >  1:1657677/1‑49 (MQ=255)                                             tgGGAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAaca  <  1:77158/49‑1 (MQ=255)                                                |                                              TGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACA  >  NC_000913/220052‑220144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑